Upregulation of Small Nucleolar RNA 78 Promotes Cell Proliferation and Invasion of Breast Cancer

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Abstract OBJECTIVE: Recent studies suggest that abnormal snoRNA expression may play crucial role in tumor development and progression. In the study, we aim to explore the clinical significance and functional role of Small nucleolar RNA 78 (SNORD78) in breast cancer.PATIENTS AND METHODS: The expression of SNORD78 in 92 breast cancer and adjacent non tumor tissues was analyzed by quantitative real time polymerase chain reaction (qRT-PCR). The association between SNORD78 expression and clinicopathological factors was evaluated. The biological functions in vitro were examined by MTT cell proliferation, colony formation and transwell invasion assays. QRT-PCR and Western blot assays were used to analyze the mRNA and protein expression of WNT1, GSK3β and β-catenin.RESULTS: SNORD78 expression showed a significant increase in breast cancer tissues and cell lines. Higher SNORD78 expression positively was related to lymph node metastasis and TNM stage in patients. Furthermore, knockdown of SNORD78 inhibited the abilities of cell proliferation, colony formation, and cell invasion in MDA-MB-231 and SKBR-3 cells. Moreover, we demonstrated that knockdown of SNORD78 inhibited WNT/β-catenin signaling pathway in MDA-MB-231 and SKBR-3 cells via downregulating the relative protein expression of WNT1 and β-catenin and upregulating the GSK3β expression.CONCLUSION: Our results indicated that SNORD78 may be potential therapeutic target for breast cancer.

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