Transplantation - basic

Le résumé fourni par la source

Introduction and Aims:Cyclosporine A (CsA) is often used immunosuppresive agent in the variety of clinical conditions (among other in autoimmune diseases and after organ transplantation).In utero exposure to immunosuppressive drugs may influence organogenesis of the offspring with a potentially increased risk of hypertension and CKD in adulthood.The aim of this study in rats was to assess the effect of exposure to CsA during the gestation on number and volume of glomeruli as well as of blood pressure (BP) in the offspring.Methods: Eight pregnant Sprague-Dawley rats were assigned to CsA (3 mg/kg/day s. c. from day 10 after fertilization till day 7 after delivery) or solvent (saline) respectively.BP in the offspring was measured indirectly on tail artery (CODA System, Kent Scientific, USA), during isoflurane anesthesia, on day 7 and 11 weeks after birth.12 weeks after delivery the experiment was terminated and kidneys were obtained using BP controlled glutaraldehyde perfusion.The number and volume of glomeruli was assessed using unbiased stereological methods.Results: At age 7 weeks and 11 weeks respectively in the 34 offspring of the mother rats treated with CsA systolic (SBP) and diastolic (DBP) blood pressures were higher than in the 31 offspring of pregnant rats treated with solvent (7th week -SBP: 125±5 vs. 117±6mmHg, p<0.001;DBP: 82±6 vs. 77±6mmHg, p<0.001; 11th week -SBP: 132 ±9 vs 126±7mmHg, p<0.05;DBP: 89±8 vs. 83±7mmHg, p<0.001).The number of glomeruli was lower (19,524±758 vs. 24,017±861, p<0.001) and the mean glomerular volume higher (2.44±0.32 vs. 1.68±0.19x 106μm3, <0.001) in offspring of pregnant rats treated with CsA compared to the offspring of pregnant rats treated with solvent.Conclusions: 1. Treatment with CsA during pregnancy increases blood pressure in the offspring, decreases the number of glomeruli and increases the volume of glomeruli in the offspring of rats.2. Lower number of glomeruli may participate in the pathogenesis of increased blood pressure and CKD in the adult life.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.