Metabarcoding of canopy arthropods reveals negative impacts of forestry insecticides on community structure across multiple taxa
Rattachement africain : de, ch, gb, bg. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Abstract Insecticides used to combat outbreaks of forest defoliators can adversely affect non‐target arthropods. Forestry insecticides typically suppress Lepidoptera larvae which are the cornerstone of the canopy community of deciduous oak forests. The abrupt removal of this dominant component of the food web could have far‐reaching implications for forest ecosystems, yet it is rarely investigated in practice owing to several methodological shortcomings. The taxonomic impediment and the biased nature of arthropod sampling techniques particularly impede the assessment of insecticide impacts on diverse communities. To tackle this issue, we propose an experimental approach combining sampling by pyrethrum knockdown and species determination via DNA metabarcoding, using community subsampling to derive estimates of species abundances. We applied this protocol to investigate the short‐term effects of the insecticides diflubenzuron (DFB) andBacillus thuringiensisvar.kurstaki(BTK) on canopy‐dwelling arthropod communities in German oak woodlands. Our approach allowed us to detect most of the diversity and integrate species abundances in our analyses. By classifying arthropod species into assemblages based on their expected sensitivity rather than coarse taxonomic groupings, we could unveil substantial effects of DFB across multiple taxa 5 weeks after application. Although strong effects on single species appear related to direct toxicity, substantial impacts of DFB on parasitoids and xylophagous beetles suggest that anti‐defoliator treatments can have previously unsuspected indirect effects on some components of forest arthropod communities. The impacts of BTK on community structure were consistent with that of DFB though much weaker. Synthesis and applications. Comparing diversity patterns in the arthropod communities of sprayed and unsprayed oak canopies, our results show that selective insecticides can alter species diversity in presumably non‐sensitive taxa. Even though the ecological significance of these impacts has yet to be assessed in an operational setting, their existence calls for increased regulatory scrutiny on indirect effects. As community approaches become more attainable with the rapid development of DNA metabarcoding, we suggest the inclusion of community‐level end points as regulatory requirements for the approval of forest‐use insecticides.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Metabarcoding of canopy arthropods reveals negative impacts of forestry insecticides on community structure across multiple taxa
- Date Crossref
- 30/01/2022
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Weihenstephan-Triesdorf University of Applied Sciences pays non établi dans la noticeUniversité ou école supérieure
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Technical University of Munich Terrestrial Ecology Research Group pays non établi dans la noticeUniversité ou école supérieure
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ABX-CRO Advanced Pharmaceutical Services (Germany) pays non établi dans la noticeEntreprise
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Bern University of Applied Sciences pays non établi dans la noticeUniversité ou école supérieure
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University of Würzburg pays non établi dans la noticeUniversité ou école supérieure
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Swiss Federal Institute for Forest pays non établi dans la noticeStructure de recherche
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University of Liverpool Department of Evolution pays non établi dans la noticeUniversité ou école supérieure
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Bavarian State Research Center for Agriculture pays non établi dans la noticeOrganisme public
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Forest Research Institute pays non établi dans la noticeStructure de recherche
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School of Life Sciences Weihenstephan Terrestrial Ecology Research Group pays non établi dans la noticeUniversité ou école supérieure
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AIM—Advanced Identification Methods GmbH Leipzig Germany pays non établi dans la noticeEntreprise
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Forest Entomology Swiss Federal Research Institute WSL Birmensdorf Switzerland pays non établi dans la noticeStructure de recherche
Weihenstephan-Triesdorf University of Applied Sciences, Terrestrial Ecology Research Group — Technical University of Munich et ABX-CRO Advanced Pharmaceutical Services (Germany), avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.