In silico analysis of bovine Glutathione-S-transferase M1 (GSTM1), an essential gene governing bull semen quality

Résumé fourni par la source

Artificial insemination has great applicability in the dairy industry globally.However, poor semen quality with subfertility in bulls is a major challenge to increase the conception rate following insemination.Glutathione-S-transferase M1 (GSTM1) is an important gene that regulates semen quality in a bull.The bovine GSTM1 protein sequence was retrieved; its three-dimensional structure was predicted and validated in silico in this study.The three-dimensional protein structure was predicted using Modeller 9.24, I-TASSER, Phyre 2 and Swiss Model.Structure validation was done by observing the Ramachandran Plot, Hydrophobicity Plot considering amino acid residues in the favored region and several other tools.The model predicted by Modeller 9.24 was the best model with 98.1% residues in the favored region, 0.5% in the allowed region and 1.4% in the outlier region.The predicted structure was docked with glutathione as ligand using PatchDock, and docked poses were analyzed using Ligplot plus.Interactomic analysis with STRING database reveals its interaction with other variants of Glutathione-S-transferase and cytochromeP450 family genes.Prediction of the structure of GSTM1 protein will improve our understanding of its function to determine bull semen quality.Also, the study will be a significant theoretical resource to conduct future studies aiming to develop candidate gene markers for selecting superior quality bulls.

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