Produced by Crossing Albino Sea Cucumbers Apostichopus japonicus

Le résumé fourni par la source

Body color is an economic trait of Apostichopus japonicus with growing concern. Among the other color variants (green and purple), rare sea cucumbers with completely white body color is greatly appealing to the consumers. In this study, comparative transcriptome analysis of body wall from body-color segregated progenies produced by cross of albino sea cucumber bmodstock was performed using RNA-Seq technology in order to profile dynamic characteristics of genome-wide gene expression. Over 4.78 and 4.87 million clean reads were generated from the segregated and albino juveniles crossed by albino parent sea cucumbers. Totally 20,319 assembled unigenes with the average 959.83 bp in length were obtained, including the annotated 7,152. Compared with white juvenile sea cucumbers, 271 genes were validated to be significantly differentially expressed in the body wall of segregated samples. Forty-two differentially expressed genes (DEGs) including 31 upregulated and 11 downregulated were identified presenting 5 and more fold expression changes, 18 of which were involved in growth and development, immunity and respiration. Cell and cell part, Cellular process and Binding were the most DEG-enriched GO terms in Cell component, Biological process and Molecular function, respectively. DEGs were significantly enriched in immunity-related KEGG pathways, such as Phagosome, Staphylococcus aureus infection, Complement and coagulation cascades, and Vibrio cholerae infection. These results provided abundant candidate genes and pathways to further reveal differences in growth and immunity between segregated and white progenies crossed from albino A. japonicus, for cultivating genetically stable white strain of this commercially important species. (C) 2020 Friends Science Publishers

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