Determination of combination performance of Elisa test and Western Blot for pig diagnostic in Côte d’Ivoire

Le résumé fourni par la source

Serological diagnosis of cysticercosis allows a detection of the disease on living pigs. It routinely uses Elisa as a screening test and Western blot (Eitb) as a confirmatory test. The aim of this study was to assess the performance of the Elisa / Eitb association in order to improve decision-making for the control of this pathology in Cote d’Ivoire. A group of 246 of samples of pigs serums, divided into 123 negative for Elisa and 123 positive for Elisa were drawn at random and were analyzed by the Western blot test. Thus, a contingency table was used to analyze the characteristics of the screening test (Elisa) through the parameters of sensitivity (Sp), specificity (Sp). These performances of diagnostic of the combination of Elisa / Eitb tests was evaluated according to texts design in serial or mixed-strategy. The data obtained for these two patterns were compared. The overall results showed good sensitivity (Se = 76.2%) with average specificity (Sp = 55.4%). The diagnostic performance evaluation of the combination of Elisa / Eitb tests gave 13% serial positives and 17% in the mixed regimen, a difference of 4%. Also, on a total of 123 sreums negatives analyzed by Eitb, 10 or (8.13%) were found positives, corresponding to a loss linked to the screening of samples by Elisa.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.