A connectomic study of a petascale fragment of human cerebral cortex
Rattachement africain : ch, us, kr, mx. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Abstract We acquired a rapidly preserved human surgical sample from the temporal lobe of the cerebral cortex. We stained a 1 mm 3 volume with heavy metals, embedded it in resin, cut more than 5000 slices at ∼30 nm and imaged these sections using a high-speed multibeam scanning electron microscope. We used computational methods to render the three-dimensional structure containing 57,216 cells, hundreds of millions of neurites and 133.7 million synaptic connections. The 1.4 petabyte electron microscopy volume, the segmented cells, cell parts, blood vessels, myelin, inhibitory and excitatory synapses, and 104 manually proofread cells are available to peruse online . Many interesting and unusual features were evident in this dataset. Glia outnumbered neurons 2:1 and oligodendrocytes were the most common cell type in the volume. Excitatory spiny neurons comprised 69% of the neuronal population, and excitatory synapses also were in the majority (76%). The synaptic drive onto spiny neurons was biased more strongly toward excitation (70%) than was the case for inhibitory interneurons (48%). Despite incompleteness of the automated segmentation caused by split and merge errors, we could automatically generate (and then validate) connections between most of the excitatory and inhibitory neuron types both within and between layers. In studying these neurons we found that deep layer excitatory cell types can be classified into new subsets, based on structural and connectivity differences, and that chandelier interneurons not only innervate excitatory neuron initial segments as previously described, but also each other’s initial segments. Furthermore, among the thousands of weak connections established on each neuron, there exist rarer highly powerful axonal inputs that establish multi-synaptic contacts (up to ∼20 synapses) with target neurons. Our analysis indicates that these strong inputs are specific, and allow small numbers of axons to have an outsized role in the activity of some of their postsynaptic partners.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- A connectomic study of a petascale fragment of human cerebral cortex
- Date Crossref
- 30/05/2021
- Éditeur
- openRxiv
- Type
- posted-content
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Google (Switzerland) pays non établi dans la noticeEntreprise
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Google (United States) pays non établi dans la noticeEntreprise
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Korea Advanced Institute of Science and Technology Dept. of Bio and Brain Engineering pays non établi dans la noticeUniversité ou école supérieure
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Korea Institute of Brain Science pays non établi dans la noticeStructure de recherche
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Universidad del Noreste pays non établi dans la noticeUniversité ou école supérieure
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Harvard University pays non établi dans la noticeUniversité ou école supérieure
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Dept. of Molecular and Cellular Biology pays non établi dans la noticeInstitution
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Google Research pays non établi dans la noticeInstitution
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Northeastern University pays non établi dans la noticeUniversité ou école supérieure
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School of Engineering and Applied Sciences pays non établi dans la noticeUniversité ou école supérieure
Google (Switzerland), Google (United States) et Dept. of Bio and Brain Engineering — Korea Advanced Institute of Science and Technology, avec 7 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.