Characterization of ABH-subtype donor-specific antibodies in ABO-A-incompatible kidney transplantation
Rattachement africain : us, gb, ca. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
ABO-incompatible (ABOi) transplantation requires preemptive antibody reduction; however, the relationship between antibody-mediated rejection (AMR) and ABO-antibodies, quantified by hemagglutination (HA), is inconsistent, possibly reflecting variable graft resistance to AMR or HA assay limitations. Using an ABH-glycan microarray, we quantified ABO-A antigen-subtype (A-subtype)-specific IgM and IgG in 53 ABO-O recipients of ABO-A kidneys, before and after antibody removal (therapeutic plasma exchange [TPE] or ABO-A-trisaccharide immunoadsorption [IA]) and 1-year posttransplant. IgM binding to all A-subtypes correlated highly (R2 ≥ .90) and A-subtype antibody specificities was reduced equally by IA versus TPE. IgG binding to the A-subtypes (II–IV) expressed in kidney correlated poorly (.27 ≤ R2 ≤ .69). Reduction of IgG specific to A-subtype-II was equivalent for IA and TPE, whereas IgG specific to A-subtypes-III/IV was not as greatly reduced by IA (p < .005). One-year posttransplant, IgG specific to A-II remained the most reduced antibody. Immunostaining revealed only A-II on vascular endothelium but A-subtypes II-III/IV on tubular epithelium. These results show that ABO-A-trisaccharide is sufficient for IgM binding to all A-subtypes; this is true for IgG binding to A-II, but not subtypes-III/IV, which exhibits varying degrees of specificity. We identify A-II as the major, but importantly not the sole, antigen relevant to treatment and immune modulation in adult ABO-A-incompatible kidney transplantation.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Characterization of ABH-subtype donor-specific antibodies in ABO-A-incompatible kidney transplantation
- Date Crossref
- 01/11/2021
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Mayo Clinic Division of Nephrology and Hypertension pays non établi dans la noticeÉtablissement de santé
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NIHR Surgical Reconstruction and Microbiology Research Centre pays non établi dans la noticeStructure de recherche
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University of Alberta Department of Pediatrics pays non établi dans la noticeUniversité ou école supérieure
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James Cook University Hospital Department of Nephrology pays non établi dans la noticeÉtablissement de santé
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Alberta Glycomics Centre pays non établi dans la noticeStructure de recherche
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University of Birmingham pays non établi dans la noticeUniversité ou école supérieure
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School of Immunity and Infection pays non établi dans la noticeUniversité ou école supérieure
Division of Nephrology and Hypertension — Mayo Clinic, NIHR Surgical Reconstruction and Microbiology Research Centre et Department of Pediatrics — University of Alberta, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.