ELISPOT assay of interferon‐γ secretion for evaluating human cytomegalovirus reactivation risk in allo‐HSCT recipients
Rattachement africain : cn, Madagascar. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
T cells in HCMV-reactivated allo-HSCT recipients using an enzyme-linked immunospot (ELISPOT) assay at 3 months post-transplantation. Blood samples from 47 recipients were tested for HCMV DNAemia, HCMV pp65 antigenemia, and anti-HCMV immunoglobulins (IgG/IgM) over 3 months post-transplantation. Of the 47 transplant recipients, 26 were HLA-A*02 positive and 21 were HLA-A*02 negative. The results were essentially consistent between the 47 transplant recipients and the HLA-A*02-positive recipients. HCMV DNAemia was not linearly correlated with IFN-γ spot-forming cells (SFCs) counts; IFN-γ SFCs counts did not differ significantly between the HCMV DNAemia-positive and -negative groups, whereas the HCMV-DNA virus loads were inversely correlated with the IFN-γ SFCs counts. HCMV pp65 antigenemia was not linearly correlated with IFN-γ SFCs counts; IFN-γ SFCs counts in the HCMV pp65 antigenemia-positive and -negative groups were similar. More IFN-γ SFCs counts were detected in transplant recipients with high anti-HCMV-IgG antibody titers than in those with low anti-HCMV-IgG titers pre-transplantation in the 47 recipients. Anti-HCMV-IgG antibody titers were positively linearly correlated with IFN-γ SFCs counts in HLA-A*02-positive recipients. The HCMV infection indicators used to monitor HCMV reactivation had different values in transplant recipients. The use of the IFN-γ SFCs counts measured by ELISPOT to evaluate the risk of HCMV reactivation needs further study.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- ELISPOT assay of interferon‐γ secretion for evaluating human cytomegalovirus reactivation risk in allo‐HSCT recipients
- Date Crossref
- 09/06/2021
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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First Affiliated Hospital Zhejiang University State Key Laboratory for Diagnosis and Treatment of Infectious Diseases pays non établi dans la noticeÉtablissement de santé
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Zhejiang University State Key Laboratory for Diagnosis and Treatment of Infectious Diseases pays non établi dans la noticeUniversité ou école supérieure
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Tongde Hospital of Zhejiang Province pays non établi dans la noticeÉtablissement de santé
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Ministry of Public Health Madagascar (code pays fourni par la source)Organisme public
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Department of Clinical Laboratory pays non établi dans la noticeStructure de recherche
State Key Laboratory for Diagnosis and Treatment of Infectious Diseases — First Affiliated Hospital Zhejiang University, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases — Zhejiang University et Tongde Hospital of Zhejiang Province, avec 2 autres affiliations. Pays d’affiliation : Madagascar.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.