Molecular characterization and population genetics of Theileria parva in Burundi’s unvaccinated cattle: Towards the introduction of East Coast fever vaccine
Rattachement africain : Malawi, Zambie, gb. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Theileria parva (T. parva) is a protozoan parasite that causes East Coast fever (ECF). The disease is endemic in Burundi and is a major constraint to livestock development. In this study, the parasite prevalence in cattle in six regions namely; Northern, Southern, Eastern, Western, Central and North Eastern was estimated. Furthermore, the sequence diversity of p67, Tp1 and Tp2 genes was assessed coupled with the population genetic structure of T. parva using five satellite markers. The prevalence of ECF was 30% (332/1109) on microscopy, 60% (860/1431) on ELISA and 79% (158/200) on p104 gene PCR. Phylogenetic analysis of p67 gene revealed that only allele 1 was present in the field samples. Furthermore, phylogenetic analysis of Tp1 and Tp2 showed that the majority of samples clustered with Muguga, Kiambu and Serengeti and shared similar epitopes. On the other hand, genetic analysis revealed that field samples shared only two alleles with Muguga Cocktail. The populations from the different regions indicated low genetic differentiation (FST = 0.047) coupled with linkage disequilibrium and non-panmixia. A low to moderate genetic differentiation (FST = 0.065) was also observed between samples and Muguga cocktail. In conclusion, the data presented revealed the presence of a parasite population that shared similar epitopes with Muguga Cocktail and was moderately genetically differentiated from it. Thus, use of Muguga Cocktail vaccine in Burundi is likely to confer protection against T. parva in field challenge trials.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Molecular characterization and population genetics of Theileria parva in Burundi’s unvaccinated cattle: Towards the introduction of East Coast fever vaccine
- Date Crossref
- 17/05/2021
- Éditeur
- Public Library of Science (PLoS)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Centre of Ticks and Tick-borne diseases Malawi (code pays fourni par la source)Institution
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University of Zambia Zambie (code pays fourni par la source)Université ou école supérieure
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Global Alliance for Livestock Veterinary Medicines pays non établi dans la noticeOrganisation à but non lucratif
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Lilongwe Centre for Ticks and Tick-Borne Diseases Lilongwe, Malawi (pays nommé en fin d’affiliation)Organisation à but non lucratif
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School of Veterinary Medicine Department of Biomedical Sciences Lusaka, Zambie (pays nommé en fin d’affiliation)Université ou école supérieure
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International Development Research Centre Nairobi, Kenya (pays nommé en fin d’affiliation)Structure de recherche
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National Veterinary Research Laboratory Bujumbura, Burundi (pays nommé en fin d’affiliation)Structure de recherche
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LMK Medical laboratories and Consultancies Kampala, Ouganda (pays nommé en fin d’affiliation)Institution
Centre of Ticks and Tick-borne diseases (Malawi), University of Zambia (Zambie) et Global Alliance for Livestock Veterinary Medicines, avec 5 autres affiliations. Pays d’affiliation : Malawi, Zambie, Kenya, Burundi, Ouganda.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.