A comprehensive time course and correlation analysis of indomethacin-induced inflammation, bile acid alterations and dysbiosis in the rat small intestine
Rattachement africain : hu. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
It has been proposed that changes in microbiota due to nonsteroidal anti-inflammatory drugs (NSAIDs) alter the composition of bile, and elevation of hydrophobic secondary bile acids contributes to small intestinal damage. However, little is known about the effect of NSAIDs on small intestinal bile acids, and whether bile alterations correlate with mucosal injury and dysbiosis. Here we determined the ileal bile acid metabolome and microbiota 24, 48 and 72 h after indomethacin treatment, and their correlation with each other and with tissue damage in rats. In parallel with the development of inflammation, indomethacin increased the ileal proportion of glycine and taurine conjugated bile acids, but not bile hydrophobicity. Firmicutes decreased with time, whereas Gammaproteobacteria increased first, but declined later and were partially replaced by Bilophila, Bacteroides and Fusobacterium. Mucosal injury correlated negatively with unconjugated bile acids and Gram-positive bacteria, and positively with taurine conjugates and some Gram-negative taxa. Strong positive correlation was found between Lactobacillaceae, Ruminococcaceae, Clostridiaceae and unconjugated bile acids. Indomethacin-induced dysbiosis was not likely due to direct antibacterial effects or alterations in luminal pH. Here we provide the first detailed characterization of indomethacin-induced time-dependent alterations in small intestinal bile acid composition, and their associations with mucosal injury and dysbiosis. Our results suggest that increased bile hydrophobicity is not likely to contribute to indomethacin-induced small intestinal damage.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- A comprehensive time course and correlation analysis of indomethacin-induced inflammation, bile acid alterations and dysbiosis in the rat small intestine
- Date Crossref
- 01/08/2021
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Semmelweis University Department of Pharmacology and Pharmacotherapy pays non établi dans la noticeUniversité ou école supérieure
-
University of Veterinary Medicine Department of Ecology pays non établi dans la noticeUniversité ou école supérieure
-
Pharmahungary Group (Hungary) pays non établi dans la noticeEntreprise
-
Pázmány Péter Catholic University pays non établi dans la noticeUniversité ou école supérieure
-
University of Pecs Department of Medical Biology pays non établi dans la noticeUniversité ou école supérieure
-
Faculty of Information Technology and Bionics pays non établi dans la noticeUniversité ou école supérieure
Department of Pharmacology and Pharmacotherapy — Semmelweis University, Department of Ecology — University of Veterinary Medicine et Pharmahungary Group (Hungary), avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.