The phosphoproteome of rice leaves responds to water and nitrogen supply
Rattachement africain : au, sa, dk. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Abstract The scarcity of freshwater is an increasing concern in flood-irrigated rice, whilst excessive use of nitrogen fertilizers is both costly and contributes to environmental pollution. To co-ordinate growth adaptation under prolonged exposure to limited water or excess nitrogen supply, plants have processes for signalling and regulation of metabolic processes. There is limited information on the involvement of one of the most important post-translational modifications (PTMs), protein phosphorylation, on plant adaptation to long-term changes in resource supply. Oryza sativa cv. Nipponbare was grown under two regimes of nitrogen from the time of germination to final harvest. Twenty-five days after germination, water was withheld from half the pots in each nitrogen treatment and low water supply continued for an additional 26 days, while the remaining pots were well watered. Leaves from all four groups of plants were harvested after 51 days in order to test whether phosphorylation of leaf proteins responded to prior abiotic events. The dominant impact of these resources is exerted in leaves, where PTMs have been predicted to occur. Proteins were extracted and phosphopeptides were analysed by nanoLC-MS/MS analysis, coupled with label-free quantitation. Water and nitrogen regimes triggered extensive changes in phosphorylation of proteins involved in membrane transport, such as the aquaporin OsPIP2-6, a water channel protein. Our study reveals phosphorylation of several peptides belonging to proteins involved in RNA-processing and carbohydrate metabolism, suggesting that phosphorylation events regulate the signalling cascades that are required to optimize plant response to resource supply.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- The phosphoproteome of rice leaves responds to water and nitrogen supply
- Date Crossref
- 16/03/2021
- Éditeur
- openRxiv
- Type
- posted-content
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Macquarie University Department of Molecular Sciences pays non établi dans la noticeUniversité ou école supérieure
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King Abdullah University of Science and Technology pays non établi dans la noticeUniversité ou école supérieure
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Australian Centre for Plant Functional Genomics pays non établi dans la noticeStructure de recherche
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The University of Adelaide Australian Plant Phenomics Facility pays non établi dans la noticeUniversité ou école supérieure
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AGRF Ltd pays non établi dans la noticeStructure de recherche
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University of Southern Denmark Department of Biochemistry and Molecular Biology pays non établi dans la noticeUniversité ou école supérieure
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King Abdullah University for Science and Technology pays non établi dans la noticeUniversité ou école supérieure
Department of Molecular Sciences — Macquarie University, King Abdullah University of Science and Technology et Australian Centre for Plant Functional Genomics, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.