Identification of KCS gene family and functional analysis of FAE-like genes from Malania oleifera
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Le résumé fourni par la source
KCS (3-ketoacyl-CoA synthase) is the key enzyme catalyzing the first step of very long chain fatty acid (VLCFA) biosynthesis. Studies showed that different KCSs possessed different substrate preference. Malania oleifera are abundance of VLCFAs in its mature seeds, especially the nervonic acid, which is essential for human health. In this study, we identified and characterized 18 KCS genes in M. oleifera genome. Phylogenetic analysis showed that these KCS genes were classified into four subfamilies, including two FAE-like, six KCS-like, eight FDH-like and two CER6. We concentrated on the functional role of two FAE-like genes, Maole003085.T1 and Maole004215.T1 which encoded predicted amino acid residues of 516 and 518 in protein, respectively. Multiple sequence alignment showed that their two proteins contained the known and conserved active sites among FAE-like subfamily. Upon heterologous expression in wild type yeast (Saccharomyces cerevisiae) INVSc1, we found that Maole004215.T1 could produce four new fatty acids including C22:0/C22:1 and C24:0/C24:1, but Maole003085.T1 only produced C22:1. Besides, upon heterologous expression in mutant yeast BY4741-△elo3, we found the Maole003085.T1 could produce C24:0 and C26:0, while the Maole004215.T1 could catalyze the formation of fatty acids C24:0, C26:0 and C28:0. These results showed Maole003085.T1 and Maole004215.T1 had fatty acid elongation activity in yeast, and possessed different substrate preference in the production of different VLCFAs. Interestingly, we found Maole004215.T1 could produce nervonic acid in yeast, which provides molecular basis on the genetic improvement and genic engineering for producing nervonic acid resources by using biotechnological methods.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Identification of KCS gene family and functional analysis of FAE-like genes from Malania oleifera
- Date Crossref
- 01/03/2021
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Tibet University pays non établi dans la noticeUniversité ou école supérieure
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Northwest University pays non établi dans la noticeUniversité ou école supérieure
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Southwest Forestry University pays non établi dans la noticeUniversité ou école supérieure
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Kunming Institute of Botany pays non établi dans la noticeStructure de recherche
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Chinese Academy of Sciences pays non établi dans la noticeOrganisme public
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Tibet Agriculture and Animal Husbandy College Department of Plant Science pays non établi dans la noticeUniversité ou école supérieure
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College of Life Science pays non établi dans la noticeUniversité ou école supérieure
Tibet University, Northwest University et Southwest Forestry University, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.