Use of Sugar Cane Molasses Enriched with Yeast Extract for the Production of Biopesticide from Bacillus thuringiensis var. kurstaki HD-1

Résumé fourni par la source

Bacillus thuringiensis subspecies kurstaki HD-1 (Btk HD-1) has been widely used for five decades as a leading biopesticide for its ecological action. However, this biopesticide often has challenges related toits costs of production and its formulation. The purpose of this work was to evaluate a novel low-cost Btk HD-1-based biopesticide production method using sugar cane molasse enriched with yeast extracts. After determining their content, Four growth media containing variable proportion of molasses and yeast extracts were formulated, then inoculated with (BtkHD-1) and incubated to 30 ± 1 °C. The results showed that the cane molasses were enriched in minerals such as Mn (142.08 mg/kg), Cu (144.1 mg/kg), Fe (108.18 mg/kg), Ca (97.2 mg/kg), K (79.7 mg/kg), Mg (73.96 mg/kg), Na (51.24 mg/kg) and Zn(17.7 mg/kg). Moreover, the quantity of yeast extracts had an impact on the growth rate and sporulation of Bt. Overall, the media with 200 g of yeast extract was the most suitable growth medium for Btk HD-1.Sugar cane molasses-based culture medium enriched with yeast extract could replace soy flour for its availability and low-cost.