Fungicidal Activity and Acute Toxicity of Three Elaborate Biopesticides Candidates for The Conservation of Fresh Kola Nuts

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Description of the Subject:The storage of fresh kola nut is a major concern in Côte d'Ivoire due to toxigenic molds proliferation.Objectives and Methods: The aim of this study is to determine, in vitro, the antifungal effect of codified biopesticides "AG", "SI" and "TB" on Aspergillus flavus, Aspergillus niger and Penicillium sp., , Aflatoxinogenic and ochratoxinogenic molds, all by ensuring their high dose safety according to the OECD guideline 423 with Wistar rats.Results: The results concerning the antifungal activity, showed that the mold strains are very sensitive to TB (inhibition rate; Ti > 75%) at the concentration of 7500 ppm, not very resistant or limited to SI (25% < Ti < 50%) and highly resistant to AG (Ti < 25%) even at high concentrations of 20000 ppm.Acute toxicity study results for biopesticides do not indicate any toxic effects at 2000 mg / kg of BW.However, at the dose of 5000 mg / kg of BW, only biopesticide TB was found biotolerated; AG and SI leading to toxic manifestations.Conclusion: Taken as a whole, our results reveal that TB has a proven antifungal property and is biotolerized even at a high dose of 5000 mg / kg BW in the rat.It could therefore be recommended as a biopesticide for the conservation of kola nuts at the use rate of 7500 ppm.