Butyrate as a bioactive human milk protective component against food allergy
Rattachement africain : it. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
BACKGROUND: Food allergy (FA) is a growing health problem worldwide. Effective strategies are advocated to limit the disease burden. Human milk (HM) could be considered as a protective factor against FA, but its mechanisms remain unclear. Butyrate is a gut microbiota-derived metabolite able to exert several immunomodulatory functions. We aimed to define the butyrate concentration in HM, and to see whether the butyrate concentration detected in HM is able to modulate the mechanisms of immune tolerance. METHODS: HM butyrate concentration from 109 healthy women was assessed by GS-MS. The effect of HM butyrate on tolerogenic mechanisms was assessed in in vivo and in vitro models. RESULTS: The median butyrate concentration in mature HM was 0.75 mM. This butyrate concentration was responsible for the maximum modulatory effects observed in all experimental models evaluated in this study. Data from mouse model show that in basal condition, butyrate up-regulated the expression of several biomarkers of gut barrier integrity, and of tolerogenic cytokines. Pretreatment with butyrate significantly reduced allergic response in three animal models of FA, with a stimulation of tolerogenic cytokines, inhibition of Th2 cytokines production and a modulation of oxidative stress. Data from human cell models show that butyrate stimulated human beta defensin-3, mucus components and tight junctions expression in human enterocytes, and IL-10, IFN-γ and FoxP3 expression through epigenetic mechanisms in PBMCs from FA children. Furthermore, it promoted the precursors of M2 macrophages, DCs and regulatory T cells. CONCLUSION: The study's findings suggest the importance of butyrate as a pivotal HM compound able to protect against FA.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Butyrate as a bioactive human milk protective component against food allergy
- Date Crossref
- 16/11/2020
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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CEINGE Biotecnologie Avanzate Franco Salvatore (Italy) pays non établi dans la noticeEntreprise
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University of Naples Federico II Department of Translational Medical Science pays non établi dans la noticeUniversité ou école supérieure
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University of Salerno Department of Medicine pays non établi dans la noticeUniversité ou école supérieure
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Ospedale Monaldi pays non établi dans la noticeÉtablissement de santé
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Regione Campania pays non établi dans la noticeOrganisme public
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MultiMedica pays non établi dans la noticeÉtablissement de santé
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Istituti di Ricovero e Cura a Carattere Scientifico pays non établi dans la noticeÉtablissement de santé
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Federico II University Hospital pays non établi dans la noticeÉtablissement de santé
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Department of Pediatric Cardiology Monaldi Hospital Naples Italy Department of Pediatric Cardiology pays non établi dans la noticeÉtablissement de santé
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Neonatal Intensive Care Unit "Betania" Evangelical Hospital Naples Italy Neonatal Intensive Care Unit pays non établi dans la noticeÉtablissement de santé
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Cardiovascular Research Unit pays non établi dans la noticeStructure de recherche
CEINGE Biotecnologie Avanzate Franco Salvatore (Italy), Department of Translational Medical Science — University of Naples Federico II et Department of Medicine — University of Salerno, avec 8 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.