Clinical relevance of plasma virome dynamics in liver transplant recipients
Rattachement africain : be, de, ir. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
BACKGROUND: The role of the microbiome in liver transplantation (LT) outcome has received a growing interest in the past decades. In contrast to bacteria, the role of endogenous viral communities, known as the virome, is poorly described. Here, we applied a viral metagenomic approach to study the dynamic evolution of circulating viruses in the plasma of LT recipients and its effect on the clinical course of patients. METHODS: Patients chronically infected with hepatitis B virus (HBV) that received a LT due to endstage liver disease were included in this study. Longitudinal plasma samples were collected pre- and post-LT. Intact viral particles were isolated and sequenced on an Illumina HiSeq 2500 platform. Short read libraries were analysed with an in-house bioinformatics pipeline. Key endpoints were the dynamics of viral families and post-LT complications. FINDINGS: The initiation of immunosuppression induced a bloom of the Anelloviridae that dominated the post-LT plasma virome. A variety of post-LT complication were observed. Nephrotoxicity was reported in 38% of the patients and was associated with a high abundance of anelloviruses. Besides nephrotoxicity, 16 (67%) patients experienced flares of viral or bacterial infections in post-transplant follow-up. These flares were recognized by an increased burden of anelloviruses (p < 0.05). Interestingly, no mortality was observed in patients infected with human pegivirus. INTERPRETATION: These findings suggest a diagnostic potential for the Anelloviridae family in post-LT complications. Furthermore, the impact of human pegivirus infection on post-transplant survival should be further investigated. FUNDING: This trial was supported by Gilead Sciences grant number BE-2017-000133.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Clinical relevance of plasma virome dynamics in liver transplant recipients
- Date Crossref
- 01/10/2020
- Éditeur
- Elsevier BV
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Rega Institute for Medical Research Department of Microbiology pays non établi dans la noticeStructure de recherche
-
KU Leuven pays non établi dans la noticeUniversité ou école supérieure
-
Charité - Universitätsmedizin Berlin pays non établi dans la noticeÉtablissement de santé
-
Shiraz University of Medical Sciences Department of Microbiology pays non établi dans la noticeUniversité ou école supérieure
-
High Institute for Education and Research in Transfusion Medicine pays non établi dans la noticeUniversité ou école supérieure
-
Department of Hepatology and Gastroenterology pays non établi dans la noticeInstitution
-
University Hospitals Leuven Department of Gastroenterology and Hepatology pays non établi dans la noticeUniversité ou école supérieure
-
Blood Transfusion Research Centre pays non établi dans la noticeStructure de recherche
Department of Microbiology — Rega Institute for Medical Research, KU Leuven et Charité - Universitätsmedizin Berlin, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.