MANα1‐2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV‐1
Rattachement africain : ar, it, us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
New technologies in the field of vaccinology arise as a necessity for the treatment and control of many diseases. Whole virus inactivated vaccines and modified live virus ones used against Bovine Herpesvirus-1 (BoHV-1) infection have several disadvantages. Previous works on DNA vaccines against BoHV-1 have demonstrated the capability to induce humoral and cellular immune responses. Nevertheless, 'naked' DNA induces low immunogenic response. Thus, loading of antigen encoding DNA sequences in liposomal formulations targeting dendritic cell receptors could be a promising strategy to better activate these antigen-presenting cells (APC). In this work, a DNA-based vaccine encoding the truncated version of BoHV-1 glycoprotein D (pCIgD) was evaluated alone and encapsulated in a liposomal formulation containing LPS and decorated with MANα1-2MAN-PEG-DOPE (pCIgD-Man-L). The vaccinations were performed in mice and bovines. The results showed that the use of pCIgD-Man-L enhanced the immune response in both animal models. For humoral immunity, significant differences were achieved when total antibody titres and isotypes were assayed in sera. Regarding cellular immunity, a significant increase in the proliferative response against BoHV-1 was detected in animals vaccinated with pCIgD-Man-L when compared to the response induced in animals vaccinated with pCIgD. In addition, upregulation of CD40 molecules on the surface of bovine dendritic cells (DCs) was observed when cells were stimulated and activated with the vaccine formulations. When viral challenge was performed, bovines vaccinated with MANα1-2MAN-PEG-DOPE elicited better protection which was evidenced by a lower viral excretion. These results demonstrate that the dendritic cell targeting using MANα1-2MAN decorated liposomes can boost the immunogenicity resulting in a long-lasting immunity. Liposomes decorated with MANα1-2MAN-PEG-DOPE were tested for the first time as a DNA vaccine nanovehicle in cattle as a preventive treatment against BoHV-1. These results open new perspectives for the design of vaccines for the control of bovine rhinotracheitis.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- MANα1‐2MAN decorated liposomes enhance the immunogenicity induced by a DNA vaccine against BoHV‐1
- Date Crossref
- 22/07/2020
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Consejo Nacional de Investigaciones Científicas y Técnicas pays non établi dans la noticeOrganisme public
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University of Padua pays non établi dans la noticeUniversité ou école supérieure
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Northeastern University Center for Pharmaceutical Biotechnology and Nanomedicine pays non établi dans la noticeUniversité ou école supérieure
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National Agricultural Technology Institute pays non établi dans la noticeOrganisme public
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Universidad del Salvador pays non établi dans la noticeUniversité ou école supérieure
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Instituto de Virología e Innovaciones Tecnológicas (IVIT pays non établi dans la noticeStructure de recherche
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Instituto de Investigaciones Forestales y Agropecuarias Bariloche (IFAB pays non établi dans la noticeStructure de recherche
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Department of Pharmaceutical and Pharmacological Sciences Universita degli Studi di Padova Padova PD Italy pays non établi dans la noticeInstitution
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EEA BalcarceInstituto Nacional de Tecnología Agropecuaria (INTA) Balcarce pays non établi dans la noticeStructure de recherche
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Instituto de Innovación para la Producción Agropecuaria y el Desarrollo Sostenible (IPADS) Balcarce Argentina pays non établi dans la noticeStructure de recherche
Consejo Nacional de Investigaciones Científicas y Técnicas, University of Padua et Center for Pharmaceutical Biotechnology and Nanomedicine — Northeastern University, avec 7 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.