Co-encapsulation d'un agent immunosuppresseur et d'un agent anti-inflammatoire dans une émulsion de Pickering pour le traitement topique de pathologies cutanées

Le résumé fourni par la source

In this thesis work, we formulated innovative Pickering emulsions, stabilized by biocompatible and biodegradable poly (lactic-co-glycolic acid) nanoparticles (NP), using Miglyol as the oil phase. These systems could be an alternative to conventional emulsions, often stabilized with synthetic surfactants that can be responsible for irritation in particular during long-term topical treatments. Two active pharmaceutical ingredients (API) were successfully co-encapsulated, the first one, an immunosuppressant agent, entrapped in PLGA NP, the second one, an anti-inflammatory agent, incorporated in Miglyol droplets.Firstly, a thorough physicochemical characterization allowed to better understand the stabilization mechanism of these emulsions, depending on the type of NP used. Moreover, the influence of the addition of API was evaluated on the macroscopic, microscopic and interfacial structures of the emulsions, also on their stability. Secondly, we demonstrated that the addition of Carbopol, a thickening agent, improved the stability, texture and pH of emulsions. Finally, the therapeutic potential of such system was investigated through preliminary evaluation of its cytotoxicity on keratinocyte cells, but also its immunosuppressive activity on activated immune cells and their cytokine production. Such a system could be interesting for the treatment of chronical skin diseases.

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