A novel application of Gini coefficient for the quantitative measurement of bacterial aggregation
Rattachement africain : gb, cn. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Non-surface attached bacterial aggregates are frequently found in clinical settings associated with chronic infections. Current methods quantifying the extent to which a suspended bacterial population is aggregated mainly rely on: (1) cell size distribution curves that are difficult to be compared numerically among large-scale samples; (2) the average size/proportion of aggregates in a population that do not specify the aggregation patterns. Here we introduce a novel application of Gini coefficient, herein named Aggregation Coefficient (AC), to quantify the aggregation levels of cystic fibrosis Pseudomonas aeruginosa (CF-PA) isolates in vitro using 3D micrographs, Fiji and MATLAB. Different aggregation patterns of five strains were compared statistically using the numerical AC indexes, which correlated well with the size distribution curves plotted by different biovolumes of aggregates. To test the sensitivity of AC, aggregates of the same strains were treated with nitric oxide (NO), a dispersal agent that reduces the biomass of surface attached biofilms. Strains unresponsive to NO were reflected by comparable AC indexes, while those undergoing dispersal showed a significant reduction in AC index, mirroring the changes in average aggregate sizes and proportions. Therefore, AC provides simpler and more descriptive numerical outputs for measuring different aggregation patterns compared to current approaches.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- A novel application of Gini coefficient for the quantitative measurement of bacterial aggregation
- Date Crossref
- 12/12/2019
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Southampton Institute of Life Sciences pays non établi dans la noticeUniversité ou école supérieure
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NIHR Southampton Respiratory Biomedical Research Unit pays non établi dans la noticeÉtablissement de santé
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Shanghai Jiao Tong University pays non établi dans la noticeUniversité ou école supérieure
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NIHR Southampton Respiratory Biomedical Research Centre pays non établi dans la noticeStructure de recherche
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School of Biomedical Engineering pays non établi dans la noticeUniversité ou école supérieure
Institute of Life Sciences — University of Southampton, NIHR Southampton Respiratory Biomedical Research Unit et Shanghai Jiao Tong University, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.