Determination of the frequency of β-lactamase genes (bla SHV, bla TEM, bla CTX-M) and phylogenetic groups among ESBL-producing uropathogenic Escherichia coli isolated from outpatients
Rattachement africain : ir, Soudan. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Abstract Background Escherichia coli accounts for 70–95% of community-acquired urinary tract infections (UTIs). Recently, there has been an increase in the prevalence of extended-spectrum β-lactamase (ESBL) in the community which required an accurate identification for better management. Therefore, the current study was performed to determine the antimicrobial resistance pattern, investigate ESBL phenotypes and genotypes (blaCTX-M, bla TEM and bla SHV genes) and determine the phylogenetic groups among ESBL-positive isolates from outpatients. Methods One hundred and eighty-three positive urine samples were collected from 4450 outpatient clinic attendees. Antibiotic susceptibility was determined and ESBL phenotype screening was carried out using disk diffusion agar and combination disk techniques, respectively. The assessment of the presence of the blaCTX-M, bla TEM and blaSHV genes and phylogenetic grouping were performed using the polymerase chain reaction (PCR) method. Results Out of 183 E. coli isolates, 59 (32.2%) showed a positive ESBL phenotype. The prevalence of ESBL-producing E. coli was higher in males (57.4%). Fifty-seven of the ESBL-producing strains carried at least one of the β-lactamase genes (bla CTX-M, bla TEM, bla SHV). Phylotyping of multi-drug resistant isolates indicated that the isolates belonged to B2, A and D phylogroups. Analysis of resistance patterns among these phylogroups revealed that 74.4%, 55.3% and 29.7% of the isolates in the B2 group were resistant to trimethoprim-sulfamethoxazole, ciprofloxacin and gentamicin, respectively. Most of the strains in the phylogroup B2 carried the bla CTX-M gene. Conclusions All the ESBL-producing isolates were placed in one of the four phylogenetic groups. The presence of CTX-M and resistance to quinolones were more frequent in B2 strains than in non-B2 strains.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Determination of the frequency of β-lactamase genes (<i>bla SHV</i>, <i>bla TEM</i>, <i>bla CTX-M</i>) and phylogenetic groups among ESBL-producing uropathogenic <i>Escherichia coli</i> isolated from outpatients
- Date Crossref
- 13/11/2019
- Éditeur
- Walter de Gruyter GmbH
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Iran University of Medical Sciences pays non établi dans la noticeUniversité ou école supérieure
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University of Khartoum Mycetoma Research Center University of Khartoum, Soudan (code pays fourni par la source)Université ou école supérieure
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Mycetoma Research Center Soudan (code pays fourni par la source)Structure de recherche
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Tehran University of Medical Sciences pays non établi dans la noticeUniversité ou école supérieure
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Faculty of Medicine Microbiology Department pays non établi dans la noticeUniversité ou école supérieure
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School of Public Health Department of Medical Parasitology and Mycology pays non établi dans la noticeUniversité ou école supérieure
Iran University of Medical Sciences, Mycetoma Research Center — University of Khartoum (University of Khartoum, Soudan) et Mycetoma Research Center (Soudan), avec 3 autres affiliations. Pays d’affiliation : Soudan.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.