Light-sheet modulation for increased light-efficiency in a Selective Plane Illumination Microscope

Résumé fourni par la source

Light-sheet microscopy has become one of the leading techniques for 3D in vivo imaging, thanks to its reduced photo-damaging effects on the specimen, its optical sectioning capability and its imaging speed. Various modifications of a Selective Plane Illumination Microscope (SPIM) have been proposed, with the aim to further increase 3D imaging speed, quality and resolution, and decrease photo-bleaching. We recently presented a modified SPIM, in which a Spatial Light Modulator (SLM) is used to move and modulate the light-sheet to perform different existing imaging techniques which improve image quality. We now discuss how such a system can be further exploited, and present two new light-sheet modulations specifically designed to help reduce the effect of photo-bleaching and, more in general, to improve the system’s light-efficiency.

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