MP62-20 LOCALIZATION AND EXPRESSION OF EXTRACELLULAR MATRIX COMPONENTS VERSICAN AND HYALURONAN IN URETHRAL AND VAGINAL TISSUES IN HUMAN SPECIMENS AND IN RAT MODEL OF URINARY INCONTINENCE

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You have accessJournal of UrologyUrodynamics/Lower Urinary Tract Dysfunction/Female Pelvic Medicine: Basic Research & Pathophysiology (MP62)1 Apr 2019MP62-20 LOCALIZATION AND EXPRESSION OF EXTRACELLULAR MATRIX COMPONENTS VERSICAN AND HYALURONAN IN URETHRAL AND VAGINAL TISSUES IN HUMAN SPECIMENS AND IN RAT MODEL OF URINARY INCONTINENCE Ingrid Harten, Stephen Evanko, Jay Choe, Eugene Lee, Marika Bogdani, Thomas Wight, and Una Lee* Ingrid HartenIngrid Harten More articles by this author , Stephen EvankoStephen Evanko More articles by this author , Jay ChoeJay Choe More articles by this author , Eugene LeeEugene Lee More articles by this author , Marika BogdaniMarika Bogdani More articles by this author , Thomas WightThomas Wight More articles by this author , and Una Lee*Una Lee* More articles by this author View All Author Informationhttps://doi.org/10.1097/01.JU.0000556843.34124.73AboutPDF ToolsAdd to favoritesDownload CitationsTrack CitationsPermissionsReprints ShareFacebookLinked InTwitterEmail Abstract INTRODUCTION AND OBJECTIVES: Abnormal extracellular matrix (ECM) has been correlated with stress urinary incontinence (SUI). ECM components versican (VC) and hyaluronan (HA) play key roles in regulating tissue inflammation and maintaining connective tissue homeostasis. The objective of this study is to analyze the localization and expression of ECM components VC and HA in urethral and vaginal tissues in human clinical specimens and in a rat model of urinary incontinence. METHODS: Nulliparous Sprague-Dawley female rats underwent vaginal distension (VD), a rodent model of SUI, or a sham procedure. Tissues were harvested from 6 rats per group at days 1, 4, and 21 for immunohistochemistry and RNA expression analysis of ECM components. Urethral and vaginal samples from female patients with and without SUI were also examined. RESULTS: High intensity staining of VC was found in both sham and VD animals 1 day after surgery. This elevation persisted at day 4 in VD compared to sham, with concurrent reduced mRNA expression of VC degrading enzymes ADAMST 5 and 9 (Figure 1). Abundance of HA was not different between VD and sham, however mRNA expression of the HA synthase Has2 was significantly reduced in VD at day 4. Abundant versican staining was found in 60% of patient samples with SUI, which was strongest in regions of disrupted elastin. CONCLUSIONS: Reduction of VC degrading enzymes and HA synthases at day 4 post surgery indicate a potential delay in ECM turnover associated with SUI. Abundant VC is associated with inflammation and elastin fiber network disruption, warranting further investigation to determine its role in the pathogenesis of SUI. Source of Funding: Wilske Translational Research Program, Virginia Mason Seattle, WA; San Diego, CA; Hayward, CA; Seattle, WA© 2019 by American Urological Association Education and Research, Inc.FiguresReferencesRelatedDetails Volume 201Issue Supplement 4April 2019Page: e895-e895 Advertisement Copyright & Permissions© 2019 by American Urological Association Education and Research, Inc.MetricsAuthor Information Ingrid Harten More articles by this author Stephen Evanko More articles by this author Jay Choe More articles by this author Eugene Lee More articles by this author Marika Bogdani More articles by this author Thomas Wight More articles by this author Una Lee* More articles by this author Expand All Advertisement PDF downloadLoading ...

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