ES2.3 - Precipitate-Assisted Signal Amplification of Surface Acoustic Wave Immunosensor for Mite Allergen

Le résumé fourni par la source

House dust mites (HDM) cause allergic diseases such as allergic asthma and rhinitis.In order to reduce the risk of sensitization, an airborne HDM monitoring system that is composed of a sampler and a sensor is expected to be useful.However, to meet the standard set by the World Health Organization, a high sensitivity of the sensor is required.Here, we propose a method to amplify the signal of surface acoustic wave (SAW) immunosensor for HDM allergen (Der f 2) based on a horse radish peroxidase (HRP)-mediated enzymatic reaction.3,3-diaminobenzidine (DAB) used as a substrate was polymerized through this enzymatic reaction, and a precipitate was formed.The resultant precipitate increased a total mass in close vicinity of a sensing area surface of the SAW immunosensor, thereby a signal was able to be amplified.In measurement of Der f 2 using sandwich assay with HRP-conjugated detection antibody (HRP-dAb), 3.7-fold signal amplification compared to the output signal from HRP-dAb was attained.This proposed strategy holds a potential of further signal amplification, and is expected to be useful for airborne HDM allergen monitoring.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.