Detection of HHV-6 and EBV and Cytokine Levels in Saliva From Children With Seizures: Results of a Multi-Center Cross-Sectional Study
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Le résumé fourni par la source
Background and objective: One third of children with epilepsy are refractory to medications. Growing data support a role of common childhood infections with neurotropic viruses and inflammation in epileptogenesis. Our objective was to determine the frequency of Human Herpesvirus-6 (HHV-6) and Epstein-Barr Virus (EBV) infection and cytokine levels in saliva from children with seizures compared to healthy controls and to controls with a febrile illness without seizures. Methods: In this cross-sectional multi-center study, we collected saliva from 115 consecutive children with acute seizures (cases), 51 children with a fever and no seizures or underlying neurological disease (fever controls) and 46 healthy children (healthy controls). Specimens were analyzed by a novel droplet digital PCR for HHV-6 and EBV viral DNA and a bead-based immunoassay for neuroinflammatory cytokines. Results: Cases included febrile seizures (n=30), acute seizures without (n=53) and with fever (n=4) in chronic epilepsy, new onset epilepsy (n=13), febrile status epilepticus (n=3) and first lifetime seizure (n=12). HHV-6 DNA was found in 40% of cases vs. 37% fever controls and 35% healthy controls, with no statistically significant differences. EBV DNA was also detected with no differences in 17% cases, 16% fever controls and 28% healthy controls. IL-8 and IL-1beta were increased in saliva of 32 random samples from cases compared with 30 fever controls: IL-8 cases mean (SD): 1158.07 pg/mL (1427.41); controls 604.92 (754.04); p=0.02. IL-1beta 185.76 (230.57); controls 86.99 (187.39); p=0.0002. IL-1beta level correlated with HHV6 viral load (p=0.007). Conclusion: Increase in inflammatory cytokines may play a role in the onset of acute seizures and saliva could represent an inexpensive and non-invasive method for detection of viral DNA and cytokines.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Detection of HHV-6 and EBV and Cytokine Levels in Saliva From Children With Seizures: Results of a Multi-Center Cross-Sectional Study
- Date Crossref
- 05/10/2018
- Éditeur
- Frontiers Media SA
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Children's National Emergency Medicine and Trauma Services pays non établi dans la noticeÉtablissement de santé
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George Washington University Children's National Medical Center pays non établi dans la noticeUniversité ou école supérieure
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National Institute of Neurological Disorders and Stroke Division of Neuroimmunology and Neurovirology pays non établi dans la noticeStructure de recherche
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Fondazione Santa Lucia pays non établi dans la noticeÉtablissement de santé
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University of California Multiple Sclerosis Center pays non établi dans la noticeUniversité ou école supérieure
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Clinical Epilepsy Section pays non établi dans la noticeÉtablissement de santé
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Santa Lucia Foundation Neuroimmunology Unit pays non établi dans la noticeOrganisation à but non lucratif
Emergency Medicine and Trauma Services — Children's National, Children's National Medical Center — George Washington University et Division of Neuroimmunology and Neurovirology — National Institute of Neurological Disorders and Stroke, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.