2018 article

Rapid Detection and Monitoring of Flavobacterium psychrophilum in Water by Using a Handheld, Field-Portable Quantitative PCR System

Phong Luu Nguyen, Ponnerassery S. Sudheesh, Austen C. Thomas, Mieke Sinnesael, Katherine H. Haman, Kenneth D. Cain

52Citations signalées, ce qui n’est pas une note de qualité

3Institutions déclarées

1Pays d’affiliation déclarés

Rattachement africain : us. Niveau de preuve : code pays fourni par la source.

Le résumé fourni par la source

Abstract Advances in technology are making it easier for rapid field detection of microbes in aquaculture. Specifically, real-time quantitative PCR (qPCR) analysis, which has traditionally been confined to laboratory-based protocols, is now available in a handheld, field-portable system. The feasibility of using the Biomeme handheld qPCR system for rapid (<50 min) on-site detection and monitoring of Flavobacterium psychrophilum from filtered water samples was evaluated. Paired water samples were collected over a 23-d period from microcosm tanks that housed fish injected with known levels of F. psychrophilum. Water samples were filtered through 0.45-μm nitrocellulose filters and were analyzed with both the Biomeme qPCR platform and a traditional bench qPCR protocol. The two methods identified similar fluctuations in F. psychrophilum DNA throughout the study. Standard curves relating quantification cycles to the number of F. psychrophilum colony-forming units (CFU) were constructed and analyzed; results indicated that CFU increased rapidly between days 6 and 8 of the trial and then progressively decreased during the remaining 15 d. Average calculated log10(CFU/mL) values were significantly correlated between the two platforms. Rapid, field-based qPCR can be incorporated into daily water quality monitoring protocols to help detect and monitor microbes in aquaculture systems.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.

Le contrôle bibliographique ouvert

DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.

Titre Crossref: Rapid Detection and Monitoring of <i>Flavobacterium psychrophilum</i> in Water by Using a Handheld, Field-Portable Quantitative PCR System
Date Crossref: 22/11/2018
Éditeur: Oxford University Press (OUP)
Type: journal-article

Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.

Où se fait cette recherche

Smith-Root (United States) pays non établi dans la notice
Entreprise
Florida Department of Agriculture and Consumer Services pays non établi dans la notice
Organisme public
University of Idaho Department of Fish and Wildlife Sciences and Aquaculture Research Institute pays non établi dans la notice
Université ou école supérieure
Inc. Smith-Root pays non établi dans la notice
Entreprise
Bronson Animal Disease Diagnostic Laboratory Florida Department of Agriculture and Consumer Services pays non établi dans la notice
Structure de recherche
Biomeme pays non établi dans la notice
Institution
Syndel pays non établi dans la notice
Institution

Smith-Root (United States), Florida Department of Agriculture and Consumer Services et Department of Fish and Wildlife Sciences and Aquaculture Research Institute — University of Idaho, avec 4 autres affiliations.

Une affiliation ne permet pas de déduire la nationalité d’un auteur.

Les sujets associés

Aquaculture disease management and microbiotaBacteriophages and microbial interactionsVibrio bacteria research studies

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