Assessment of the effects of levosimendan and thymoquinone on lung injury after myocardial ischemia reperfusion in rats
Rattachement africain : tr, us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
AIM: The aim of this study was to investigate the effects of levosimendan and thymoquinone (TQ) on lung injury after myocardial ischemia/reperfusion (I/R). MATERIALS AND METHODS: Twenty-four Wistar albino rats were included in the study. The animals were randomly assigned to 1 of 4 experimental groups. In Group C (control group), left anterior descending artery was not occluded or reperfused. Myocardial I/R was induced by ligation of the left anterior descending artery for 30 min, followed by 2 h of reperfusion in the I/R, I/R-levosimendan (24 µg/kg) (IRL) group, and I/R-thymoquinone (0.2 mL/kg) (IRTQ) group. Tissue samples taken from the lungs of rats were histochemically stained with H&E and immunohistochemically stained with p53, Bcl 2, Bax, and caspase 3 primer antibodies. RESULTS: Increased expression of p53 and Bax was observed (4+), especially in the I/R group. In IRTQ and IRL groups, expression was also observed at various locations (2+, 3+). H&E staining revealed that that the lungs were severely damaged and the walls of the alveoli were too thick, the number of areas examined was increased during the evaluation. Caspase 3 expression was observed to be at an (1+, 2+) intensity that was usually weak and diffuse in multiple areas. Bcl 2 was not found to be expressed in any of the tissues. H&E staining revealed that that the lungs were severely damaged in the I/R group, with the walls of the channels and alveoli thickened and edematous, and also an intense inflammatory cell migration was observed. Immunohistochemical staining was more prominent in inflammatory areas and structures around the terminal bronchioles. CONCLUSION: The findings in our study have shown that administration of levosimendan and TQ during I/R increases expression of caspase 3, p53, and Bax in lung tissue and has a protective effect on lung as distant organ. We suggest that findings of this study be elucidated with further large-scale clinical studies.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Assessment of the effects of levosimendan and thymoquinone on lung injury after myocardial ischemia reperfusion in rats
- Date Crossref
- 01/05/2018
- Éditeur
- Informa UK Limited
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Kırıkkale University pays non établi dans la noticeUniversité ou école supérieure
-
Kütahya Dumlupınar Üniversitesi pays non établi dans la noticeUniversité ou école supérieure
-
Gazi University Department of Cardiovascular Surgery pays non établi dans la noticeUniversité ou école supérieure
-
Education Training And Research pays non établi dans la noticeOrganisation à but non lucratif
-
Dr. Siyami Ersek Göğüs Kalp Ve Damar Cerrahisi Eğitim Ve Araştırma Hastanesi pays non établi dans la noticeÉtablissement de santé
-
Afyon Kocatepe University Department of Histology and Embryology pays non établi dans la noticeUniversité ou école supérieure
-
Kirikkale University Medical Faculty Department of Histology and Embryology pays non établi dans la noticeUniversité ou école supérieure
-
Dumlupinar University Medical Faculty Department of Physiology pays non établi dans la noticeUniversité ou école supérieure
-
Pediatric Cardiovascular Surgery Clinic pays non établi dans la noticeÉtablissement de santé
Kırıkkale University, Kütahya Dumlupınar Üniversitesi et Department of Cardiovascular Surgery — Gazi University, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.