COL4A5 and LAMA5 variants co-inherited in familial hematuria: digenic inheritance or genetic modifier effect?
Rattachement africain : cy, qa. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
BACKGROUND: About 40-50% of patients with familial microscopic hematuria (FMH) caused by thin basement membrane nephropathy (TBMN) inherit heterozygous mutations in collagen IV genes (COL4A3, COL4A4). On long follow-up, the full phenotypic spectrum of these patients varies a lot, ranging from isolated MH or MH plus low-grade proteinuria to chronic renal failure of variable degree, including end-stage renal disease (ESRD). METHODS: Here, we performed Whole Exome Sequencing (WES) in patients of six families, presenting with autosomal dominant FMH, with or without progression to proteinuria and loss of renal function, all previously found negative for severe collagen IV mutations. Hierarchical filtering of the WES data was performed, followed by mutation prediction analysis, Sanger sequencing and genetic segregation analysis. RESULTS: In one family with four patients, we found evidence for the contribution of two co-inherited variants in two crucial genes expressed in the glomerular basement membrane (GBM); LAMA5-p.Pro1243Leu and COL4A5-p.Asp654Tyr. Mutations in COL4A5 cause classical X-linked Alport Syndrome, while rare mutations in the LAMA5 have been reported in patients with focal segmental glomerulosclerosis. The phenotypic spectrum of the patients includes hematuria, proteinuria, focal segmental glomerulosclerosis, loss of kidney function and renal cortical cysts. CONCLUSIONS: A modifier role of LAMA5 on the background of a hypomorphic Alport syndrome causing mutation is a possible explanation of our findings. Digenic inheritance is another scenario, following the concept that mutations at both loci more accurately explain the spectrum of symptoms, but further investigation is needed under this concept. This is the third report linking a LAMA5 variant with human renal disease and expanding the spectrum of genes involved in glomerular pathologies accompanied by familial hematurias. The cystic phenotype overlaps with that of a mouse model, which carried a Lama5 hypomorphic mutation that caused severely reduced Lama5 protein levels and produced kidney cysts.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- COL4A5 and LAMA5 variants co-inherited in familial hematuria: digenic inheritance or genetic modifier effect?
- Date Crossref
- 16/05/2018
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Cyprus Medical School pays non établi dans la noticeUniversité ou école supérieure
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Archbishop Makarios III Hospital Department of Pediatric Nephrology pays non établi dans la noticeÉtablissement de santé
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Nicosia General Hospital Department of Nephrology pays non établi dans la noticeÉtablissement de santé
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Larnaca College pays non établi dans la noticeUniversité ou école supérieure
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Hippocrateon Private Hospital pays non établi dans la noticeÉtablissement de santé
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University College London Qatar pays non établi dans la noticeUniversité ou école supérieure
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Qatar University pays non établi dans la noticeUniversité ou école supérieure
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Larnaca General Hospital Department of Nephrology pays non établi dans la noticeÉtablissement de santé
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Evangelismos Hospital Department of Nephrology pays non établi dans la noticeÉtablissement de santé
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Hippocrateon Hospital pays non établi dans la noticeÉtablissement de santé
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College of Medicine pays non établi dans la noticeUniversité ou école supérieure
Medical School — University of Cyprus, Department of Pediatric Nephrology — Archbishop Makarios III Hospital et Department of Nephrology — Nicosia General Hospital, avec 8 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.