Effect of cryopreservation on lymph node fragment regeneration after autologous transplantation in the minipig model
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Le résumé fourni par la source
INTRODUCTION: Lymphoedema is a worldwide pandemic causing swelling of tissues due to dysfunctional transport of lymph fluid. Present management concepts are based in conservative palliation of symptoms through manual lymphatic drainage, use of compression garments, manual lymph drainage, exercise, and skin care. Nevertheless, some curative options as autologous lymph node transplantation were shown to reduce lymphoedema in selected cases. Lately, some concern has arisen due to reports of donor site morbidity. A possible solution could be the development of artificial lymph node scaffolds as niches of lymphatic regeneration. Engineering these scaffolds has included cryopreservation of lymph node stroma. However, the effects of cryopreservation on the regeneration capacities of these organs were unknown. MATERIALS AND METHODS: Here, we used the minipig animal model to assess lymphatic regeneration processes after cryopreservation of autologous lymph nodes. Superficial inguinal lymph nodes were excised and conserved at -80°C for 1 month. Thereafter, lymph node fragments were transplanted in the subcutaneous tissue. RESULTS: Regeneration of the lymph nodes was assessed five months after transplantation. We show that lymph node fragment regeneration takes place in spite of former cryopreservation. Transplanted fragments presented typical histological appearance. Their draining capacity was documented by macroscopic transport of Berlin Blue dye as well as through SPECT-CT hybrid imaging. DISCUSSION: In conclusion, our results suggest that processes of cryopreservation can be used in the creation of artificial lymph node scaffolds without major impairment of lymph node fragments regeneration.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Effect of cryopreservation on lymph node fragment regeneration after autologous transplantation in the minipig model
- Date Crossref
- 20/04/2018
- Éditeur
- Walter de Gruyter GmbH
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Medizinische Hochschule Hannover pays non établi dans la noticeUniversité ou école supérieure
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Practice for Lympho-Vascular Diseases Bahnhofstrasse 12 pays non établi dans la noticeInstitution
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myDent GmbH Tiergartenstrasse 114 pays non établi dans la noticeEntreprise
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Clinic of Haematology pays non établi dans la noticeÉtablissement de santé
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Clinic of Nuclear Medicine pays non établi dans la noticeÉtablissement de santé
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Institute of Functional and Applied Anatomy pays non établi dans la noticeStructure de recherche
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Institute of Immunomorphology pays non établi dans la noticeStructure de recherche
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Clinic of Plastic pays non établi dans la noticeÉtablissement de santé
Medizinische Hochschule Hannover, Practice for Lympho-Vascular Diseases Bahnhofstrasse 12 et myDent GmbH Tiergartenstrasse 114, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.