Combined Automated Measurement of Respiratory Chain Complexes and Oxidative Stress

Le résumé fourni par la source

The increasing prevalence of age-related disorders calls for innovative solutions, and mitochondrial drugs clearly have the potential to emerge as a key platform technology for the next generation of medicines. This chapter reports the development and the validation of a panel of respiratory chain complex (RCC) and oxidative stress (OS) automated assays that allow for the first time the simultaneous determination of five mitochondrial oxidative phosphorylation (OXPHOS) complexes and antioxidant and glutathione pathway enzymes in various media. OXPHOS and OS combined measurements represent a first step to metabolism integration. Understanding mitochondrial dysfunction must include general data on cell bioenergetics and on the metabolic routes that fuel mitochondrion. As compound evaluation can be performed in either preclinical or clinical stage, the development of versatile diagnostic enzyme assays usable either on a large subset of cells or tissues or on a variety of living species is strongly mandatory.

Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.