Proteomic characterisation of bovine and avian purified protein derivatives and identification of specific antigens for serodiagnosis of bovine tuberculosis
Rattachement africain : es, us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Bovine purified protein derivative (bPPD) and avian purified protein derivative (aPPD) are widely used for bovine tuberculosis diagnosis. However, little is known about their qualitative and quantitative characteristics, which makes their standardisation difficult. In addition, bPPD can give false-positive tuberculosis results because of sequence homology between Mycobacterium bovis ( M. bovis ) and M. avium proteins. Thus, the objective of this study was to carry out a proteomic characterisation of bPPD, aPPD and an immunopurified subcomplex from bPPD called P22 in order to identify proteins contributing to cross-reactivity among these three products in tuberculosis diagnosis. Trypsin digests of bPPD, aPPD and P22 were analysed by nanoscale liquid chromatography-electrospray ionization tandem mass spectrometry. Mice were immunised with bPPD or aPPD, and their serum was tested by indirect ELISA for reactivity against these preparations as well as against P22. A total of 456 proteins were identified in bPPD, 1019 in aPPD and 118 in P22; 146 of these proteins were shared by bPPD and aPPD, and 43 were present in all three preparations. Candidate proteins that may cause cross-reactivity between bPPD and aPPD were identified based on protein abundance and antigenic propensity. Serum reactivity experiments indicated that P22 may provide greater specificity than bPPD with similar sensitivity for ELISA-type detection of antibodies against M. tuberculosis complex. The subpreparation from bPPD called P22 may be an alternative to bPPD for serodiagnosis of bovine tuberculosis, since it shares fewer proteins with aPPD than bPPD does, reducing risk of cross-reactivity with anti- M. avium antibodies.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Proteomic characterisation of bovine and avian purified protein derivatives and identification of specific antigens for serodiagnosis of bovine tuberculosis
- Date Crossref
- 02/11/2017
- Éditeur
- Springer Science and Business Media LLC
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Universidad Complutense de Madrid pays non établi dans la noticeUniversité ou école supérieure
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Instituto de Salud Carlos III Centro Nacional de Microbiología pays non établi dans la noticeOrganisation à but non lucratif
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Centro Nacional de Microbiologia pays non établi dans la noticeStructure de recherche
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Instituto de Investigación en Recursos Cinegéticos pays non établi dans la noticeStructure de recherche
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Asociación de Investigación Metalúrgica del Noroeste pays non établi dans la noticeOrganisation à but non lucratif
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Centro de Investigación del Cáncer pays non établi dans la noticeStructure de recherche
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Oklahoma State University Department of Veterinary Pathobiology pays non établi dans la noticeUniversité ou école supérieure
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Vodafone (Spain) pays non établi dans la noticeEntreprise
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VISAVET Health Surveillance Centre pays non établi dans la noticeInstitution
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CZ Veterinaria S.A pays non établi dans la noticeInstitution
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Instituto de Biología Molecular y Celular del Cáncer-USAL-CSIC Unidad de Proteómica pays non établi dans la noticeStructure de recherche
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Instituto de Biología Molecular y Celular del Cáncer—USAL-CSIC Unidad de Proteómica pays non établi dans la noticeStructure de recherche
Universidad Complutense de Madrid, Centro Nacional de Microbiología — Instituto de Salud Carlos III et Centro Nacional de Microbiologia, avec 9 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.