P4042 Design of a polymorphic microsatellite set for domestic turkey (meleagris gallopavo) genetic characterization

Résumé fourni par la source

The ancestors of the domestic turkey (Meleagris gallopavo) evolved from an ancestor who crossed the Bering Strait when Alaska was connected with Eurasia. The domestic turkey is nowadays characterized by a strong intensive rearing system using only a few genetic improved lines, whereas local breeds are severely endangered. Local turkey populations can be found in several countries such as Mexico, Spain and Italy, and several Islamic countries (Iran, Egypt and Nigeria). In Mexico, this species has a great socio-cultural role and can be found in towns and suburban areas where low production system characterized by poor sanitary practices and unbalanced diets are common. Generally, rural people of such countries use this production in traditional parties or special events such as Christmas, the New Year or birthdays. These genetic resources are not properly kneed, and the lack of scientific information could lead to the extinction of the local breeds of important example of genetic variability and environmental adaptation. Although there are several microsatellites markers designed for mapping or QTLs search purposes, there is no a standard panel marker for genetic characterization and genetic diversity assessment. For this reason, the aim of this study is to design a polymorphic set of microsatellites that can be used for population study with the future view to perform a world study of local turkey’s populations. Thirty microsatellites were selected according to the availability polymorphism information, DNA sequences, chromosomal position completeness and quality of repeat motif: WT83, TUM20, MNT318, RHT024, MNT374, WT90, MNT331, MNT353, MNT288, MNT264, MNT258, MNT360 MNT379, MNT391, RHT0009, MNT247, MNT393, MNT266, MNT411, MNT274, MNT295, MNT348, MNT297, MNT386, MNT282, RHT0216, MNT321, MNT294, WT54 and MNT389. We used the M13-tailed primer method (Boutin-Ganache et al., 2001) to label amplicons for visualization on the capillary sequencer allowing allocate all loci in a unique electrophoresis set using Applied Biosystems G5 dye set. The primer sequence was redesigned when possible to achieve an optimal annealing temperature and amplification product size compatibility. The loci were tested on 50 DNA samples from commercial and local turkey breeds. All markers were amplified in a total number of six multiplex and were polymorphic, showing a number of alleles ranging from 3 to 14.

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