Effect of surface functional groups on chondrocyte behavior using molecular gradients

Le résumé fourni par la source

Osteoarthritis (OA) is the most common articular disease and the most prevalent condition resulting in disability among the United States adult population.According to the U.S. Department of Health and Human Services, from 2010-2012, 52.5 million (22.7%) of adults aged ≥18 years had self-reported doctor-diagnosed arthritis, and 22.7 million (9.8%) reported arthritis-attributable activity limitation, which indicates not only an ethical, but also economic importance of this disease.OA is characterized by progressive loss of articular cartilage and leads to chronic pain and functional restrictions in the affected joint.Although current treatments are successful in some aspects to provide short-term pain relief and recovered joint mobility, their long term benefits remain elusive and there is still no cure for the disease.The limited capacity for treatment is mainly due to the cartilage`s inability to repair itself.Regenerative medicine using tissue-engineered cartilage has the potential to address this issue, but a remaining challenge is the development of a feasible large scale cell expansion process, since during the expansion in monolayer cultures, chondrocytes undergo the process of dedifferentiation.Several surface-engineering approaches with bioactive factors and surface chemistry have been previously studied to look at increasing the interfacial interaction between the materials and cells.This project aimed to study the effects of various concentrations of surface functional groups on chondrocyte behavior.The cell proliferation and phenotype maintenance within continuously variable one-dimensional concentration gradients were examined.This iv method included fabrication of functionalized gradients by a vapor deposition technique that provided a fast, efficient, and reliable strategy by incorporating a series of concentrations in single substrates.Finally, human primary chondrocytes density and cellular survival were studied as response of amine and hydroxyl terminal groups` concentrations.

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