PARENTAGE TESTING IN DIFFERENT BREEDS OF DOGS USING MICROSATELLITE MARKERS

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ABSTRACT A total of 45 blood samples from progeny and the putative parents were tested for parentage analysis using a panel of microsatellite markers (short tandem repeats). Six breeds of dogs were represented viz. German shepherd, Dalmatian, Labrador, Great Dane, Boxer and Lhasa Apso in this study for genotype analysis. Ten different microsatellite marker loci were amplified by multiplex PCR. The multiplex PCR products were run in genetic analyzer. The data were analyzed using gene mapper software to measure the allele size. Such measured allele size was compared to determine whether there are matches between the progeny and the putative parents. In conclusion, short tandem repeats uniformly distributed in the genome was found to be highly polymorphic and can be used as molecular tool for parentage testing in dogs of different breeds. Key words: microsatellite markers, dog parentage, multiplex PCR.. REFERENCES Hearne, C.M., Ghosh, S., Todd J.A.1992. Microsatellites for linkage analysis of genetic traits. Trends genet. 8:288–294. Ichikawa, Y. Takagi, K. Tsumagari, S. Ishihama, K. Morita, M. Kanemaki, M. Takeishi, M. and Takahashi, H. (2001). Canine parentage testing based on microsatellite polymorphisms. J vet. Med. sci. 63:1209-1213. Jeffreys, A.J. Wilson, V. and Thein, S.L. (1985): Individual-specific fingerprints of human DNA. Nature, 316(6023):76–79. Muller, S. Flekna, G., Muller, M., Brem, G. 1999. Use of canine microsatellite polymorphisms in forensic examinations. J. Hered. 90:55-56. Nakamura, Y., Leppert, M., O'Connell, P., Wolff, R., Holm, T., Culver, M., Martin, C., Fujimoto, E., Hoff, M., Kumlin, E. 1987. Variable number of tandem repeat (VNTR) markers for human gene mapping. Science. 235.1616-22. Ostrander, E.A. and Robert K. W. 2005. The canine genome. Genome Res. 15: 1706-1716. Stallings, R.L., Ford, A.F., Nelson, D., Torney, D.C., Hildebrand, C.E., Moyzis, R.K. 1991. Evolution and distribution of (GT)n repetitive sequences in mammalian genomes. Genomics. 10(3):807-15. Walsh, P.S., Fildes, N.J., Reynolds, R. 1996. Sequence analysis and characterization of stutter products at the tetranucleotide repeat locus vWA. Nucleic Acids Res. 24: 2807–2812. Zaje, I. 1994. A new method of paternity testing for dogs, based on microsatellite sequences. Vet. Rec. 135: 545-547.

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