Abstract P311: CAPTURE: Cardio-Pulmonary Tissue Repository

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Background: Cardiothoracic tissue is highly sought by researchers, yet repositories for such tissue are conspicuously absent in the U.S.A. This limits the availability of research-quality tissue specimens. Columbia University is one of the nation's busiest heart and lung transplant centers and has excellent access to such tissue. Ca rdio p ulmonary T iss u e Re pository (CAPTURE) aims to fill this existing resource gap by creating a large-scale cardiothoracic tissue repository. Methods: Since 1999 Columbia University has collected cardiothoracic tissue on an informal basis. This is now being transitioned into CAPTURE, a sophisticated tissue bank for the collection, preservation, and distribution of research-quality tissue specimens. A team is on call 24/7 to collect tissue samples and process them immediately by flash freezing, formalin or RNAlater. Samples are annotated with clinical data from the Patients' Advantage Cardiothoracic Surgery Clinical Research Project (PACTS-CR Project), labeled with bar codes, catalogued using PROGENY LIMS (a secure electronic tracking system), and stored in freezers protected by emergency back-up ( centralized storage and collection ). CAPTURE aims to expand into a strong network of regional hospitals that contribute and utilize tissues ( centralized storage and decentralized collection ), and then to transition into a specialized program of research excellence (SPORE), becoming an NIH/NHLBI partner and later a nationwide cardiopulmonary tissue repository ( decentralized storage and collection ). Results: Tissue has been obtained from over 700 patients over the last decade. By obtaining up to 35 samples from each heart explanted during orthotopic heart transplant and up to 10 samples per myocardial core taken during ventricular assist device (VAD) implantation, over 12,000 tissue samples are currently stored. Intra- and multicenter collaborations have utilized these tissues to conduct several different studies. Conclusion: Successful past experiences have shown the promise of maintaining even an informal bank of cardiothoracic tissue samples. By offering greatly expanded and sophisticated tissue banking facilities, we anticipate CAPTURE will accelerate basic science and translational research.

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