Vitamin C Inhibits Aggravated Eryptosis by Hydrogen Peroxide in Glucose-6-Phosphated Dehydrogenase Deficiency
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Le résumé fourni par la source
BACKGROUND/AIMS: The study was aimed to investigate if vitamin C could exert protective effects on development of eryptosis caused by glucose-6-phosphate dehydrogenase (G6PD) deficiency and hydrogen peroxide. METHODS: Isolated erythrocytes with different G6PD activity (normal or deficient) were divided into various groups treated by either Vitamin C or H2O2. Phosphatidylserine (PS) extroversion rate was detected by Annexin V binding. The intracellular Ca2+ concentration was detected by Fluo3-fluorescence, and western blot was used to detect the expression of apoptosis factor caspase 3. RESULTS: Compared with the blank group, the PS extroversion rate (P < 0.001), intracellular Ca2+ concentration (P < 0.001) and active caspase 3 expression level (P < 0.05) of erythrocytes significantly increased after treatment of 0.05% H2O2. Then the index of eryptosis significantly decreased after erythrocytes were treated with Vitamin C (1 mg/ml) for 30 min (all P < 0.05). The decline in erythrocytes with G6PD normal activity was more significant than those with G6PD deficiency. CONCLUSION: Vitamin C could effectively inhibit the eryptosis contributed by H2O2 oxidative stress, and the suppression on eryptosis with G6PD normal activity was more effective than that with G6PD deficiency.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Vitamin C Inhibits Aggravated Eryptosis by Hydrogen Peroxide in Glucose-6-Phosphated Dehydrogenase Deficiency
- Date Crossref
- 01/01/2016
- Éditeur
- S. Karger AG
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Affiliated Hospital of Jining Medical University Department of Laboratory pays non établi dans la noticeÉtablissement de santé
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Yidu Central Hospital of Weifang Department of Hyperbaric Oxygen pays non établi dans la noticeÉtablissement de santé
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Third People's Hospital of Hefei pays non établi dans la noticeÉtablissement de santé
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First People's Hospital of Xianyang Intensive Care Unit pays non établi dans la noticeÉtablissement de santé
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Department of Laboratory pays non établi dans la noticeStructure de recherche
Department of Laboratory — Affiliated Hospital of Jining Medical University, Department of Hyperbaric Oxygen — Yidu Central Hospital of Weifang et Third People's Hospital of Hefei, avec 2 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.