Design of suitable carrier buffer for free‐flow zone electrophoresis by charge‐to‐mass ratio and band broadening analysis
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Le résumé fourni par la source
In this work, charge-to-mass ratio (C/M) and band broadening analyses were combined to provide better guidance for the design of free-flow zone electrophoresis carrier buffer (CB). First, the C/M analyses of hemoglobin and C-phycocyanin (C-PC) under different pH were performed by CLC Protein Workbench software. Second, band dispersion due to the initial bandwidth, diffusion, and hydrodynamic broadening were discussed, respectively. Based on the analyses of the C/M and band broadening, a better guidance for preparation of free-flow zone electrophoresis CB was obtained. Series of experiments were performed to validate the proposed method. The experimental data showed high accordance with our prediction allowing the CB to be prepared easily with our proposed method. To further evaluate this method, C-PC was purified from crude extracts of Spirulina platensis with the selected separation condition. Results showed that C-PC was well separated from other phycobiliproteins that have similar physicochemical properties, and analytical grade product with purity up to 4.5 (A620/A280) was obtained.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Design of suitable carrier buffer for free‐flow zone electrophoresis by charge‐to‐mass ratio and band broadening analysis
- Date Crossref
- 28/07/2016
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Shanghai Jiao Tong University Laboratory of Analytical Biochemistry and Bio‐separation pays non établi dans la noticeUniversité ou école supérieure
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Bioscience (China) pays non établi dans la noticeEntreprise
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South China University of Technology pays non établi dans la noticeUniversité ou école supérieure
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School of Life Science and Biotechnology Laboratory of Analytical Biochemistry and Bio-separation pays non établi dans la noticeUniversité ou école supérieure
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These authors contributed equally to this work pays non établi dans la noticeInstitution
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School of Bioscience and Bioengineering pays non établi dans la noticeUniversité ou école supérieure
Laboratory of Analytical Biochemistry and Bio‐separation — Shanghai Jiao Tong University, Bioscience (China) et South China University of Technology, avec 3 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.