Analysis on Dynamic Expression of Gene mRNA Related with Hormone in HPA and HPT Axis

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Wanxi White geese were used to study the effects on the dynamic expression of TRH,TSH,CRH and ACTH mRNA in HPA and HPT axis under cold stress(12±1)℃ by FQ-RT-PCR.Hypothalamic TRH mRNA transcription level increased significantly at 0.5 h,3 h,6 h,9 h and 18 h during cold stress.It increased again after cold exposure in 1 h,6 h and 12 h,and decreased to normal level in the rest time.Pituitary TSH mRNA transcription level was increased significantly from 1.5 h to 12 h,moreover,it reached the highest level at 12 h,then it declined to normal level.It increased again after cold exposure from 1 h to 6h and was decreased to normal level after 6h.Hypothalamic CRH mRNA transcription level increased significantly at 3 h,9 h,18 h during cold stress and at 1 h,6 h and 12 h after cold exposure.It was down-regulated to normal level at the rest time.Adrenal gland ACTH gene mRNA transcription level increased significantly from 1.5 h to 9 h and at 18 h during cold stress,and increased again after cold exposure at 0.5 h and 3 h.Hypothalamic TRH mRNA transcription level had fluctuation trend and it's quantity was not over 1.5,which indicated that hypothalamic TRH was the down-regulation gene in cold stress reaction.The transcription quantity of TSH,CRH and ACTH was 2.27-7.76、1.75-6.17、5.5-14.83 and had ascend trend,which showed that they were the up-regulation gene in cold stress reaction.The results demonstrated that TRH,TSH,CRH and ACTH gene could be probably used as the molecular biomarker of animal cold stress.

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