Chemical Composition and Fumigation Activity of Essential Oils from Leaves of Two Species of Pinaceae against Mosquitoes

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Essential oils were extracted from fresh leaves of Cedrus deodara and Pinus massoniana by steam distillation.The fumigating insecticidal activities of C.deodara oil and P.massoniana oil against Aedes albopictus and Culex quinquefasciatus at different dosages were measured by airtight cylinder method.And,the chemical components of essential oils were analyzed and determined by gas chromatography-mass spectrumetry.The LC 50 values of C.deodara oil and P.massoniana oil against adult of A.albopictus and C.quinquefasciatus at 24 h were 4.23,2.32 μg.cm 3 and 25.62,23.02 μg.cm 3,respectively.The KT 50 values were 15.50,13.69 min and 17.77,17.23 min at the concentration of essential oils of LC 50 values at 24 h.Twenty five compounds were identified in C.deoara oil,including myrcene(30.45%),α pinene(29.04%),β pinene(16.49%),α terpineol(9.29%),caryophyllene(4.09%).Thirty three compounds were identified in P.massoniana oil,including β pinene(41.95%),α pinene(22.78%),β phellandrene(5.03%) and germacrene d(4.81%).The results indicate that C.deodara oil and P.massoniana oil are of high fumigation toxicity to mosquitoes and have the potential to be developed as effective,environment-friendly insecticide for control of mosquitoes.

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