Expression and relationship of Survivin gene and cyclin D1 in human osteosarcoma

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OBJECTIVE:To explore the expression and relationship of anti-apoptosis gene Survivin and cyclin D1 in osteosarcoma in human.METHODS: The in situ hybridization, immunohistochemistry and DNA in situ end-labeling (TUNEL) methods were adopted to detect the expression of Survivin mRNA in 38 osteosarcoma samples 15 osteochondroma samples and 5 normal ones. The expression of cyclin D1 and apoptosis in osteosarcoma were detected. At the same time the expression of Survivin mRNA with the main parameters of clinical pathology and cyclin D1 expression in osteosarcoma was compared.RESULTS: Expression of Survivin was rarely seen in ostechondroma and normal osseous tissues, but significantly expressed in osteosarcoma (65.8%,25/38). The expression of Survivin was not correlated with osteosarcoma histology grade but significantly correlated with WHO typing and metastasis,P0.05. Apoptosis cells were detected in osteosarcoma, and the mean value of AI was 8.3%(0-17%). AI of negative group of Survivin was significantly higher than that of positive group,P0.01. Protein of cyclin D1 was overexpressed in osteosarcoma (71.1%,27/38), and its expression had positive correlation with expression of Survivin,r=0.370,P0.05.CONCLUSIONS: Survivin selectively expresses in osteosarcoma, by suppressing apoptosis in combination with cyclin D1 it may involved in the etiology of osteosarcoma. Survivin is possibly an important reference marker in evaluating the prognosis of osteosarcoma.

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