Role of Innate and Acquired Immune Mechanisms in Clinical Intestinal Transplant Rejection
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Le résumé fourni par la source
BACKGROUND: Long-term outcomes of intestinal transplantation are limited by infection and rejection. To understand the underlying immune mechanisms, graft infiltrating and peripheral blood cells were analyzed using multiple ex vivo assays in intestinal transplantation recipients. METHODS: Infiltrating cells from rejected (graft enterectomy for rejection) and accepted or quiescent (stoma closure in stable transplant recipients) grafts were isolated and phenotypically characterized as to subsets and Toll-like receptor expressions as well as functionally tested for antimicrobial and antidonor immune responses. Multiparameter antidonor immunity was also assessed serially in the peripheral blood. RESULTS: The graft infiltrating lymphocytes were mostly of recipient origin in all patients tested. In rejecting grafts, the predominant populations were TcRαβ(+)CD3(+)CD8(+) T cells, and CD14(+) monocytes that coexpressed Toll-like receptor-2, receptor-3, receptor-4, receptor-5, and receptor-9, suggesting innate immune activation. In quiescent allografts the major cell subsets were CD13(+)CD14(-) monocytes and CD4(+)CD25(+) T cells with possible regulatory functions. Infiltrating cells from rejected but not quiescent grafts proliferated in response to enteric bacterial and donor antigens as well as killed donor targets. Serial follow-up of peripheral blood indicated donor-specific posttransplant unresponsiveness in micro-cell-mediated lympholysis (m-CML) and mixed lymphocyte reaction (MLR) in recipients with quiescent grafts, but not in recipients with multiple rejection episodes. Enzyme-Linked ImmunoSpot assays yielded parallel results: granzyme-B with micro-cell-mediated lympholysis and interferon-γ with MLR tests. CONCLUSION: These results were consistent with the notion that rejection was associated with innate and acquired antimicrobial and antidonor immune reactivity and that patients with stable grafts were free from these deleterious effects.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Role of Innate and Acquired Immune Mechanisms in Clinical Intestinal Transplant Rejection
- Date Crossref
- 01/06/2015
- Éditeur
- Ovid Technologies (Wolters Kluwer Health)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Northwestern University Comprehensive Transplant Center pays non établi dans la noticeUniversité ou école supérieure
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University of Miami Department of Microbiology-Immunology pays non établi dans la noticeUniversité ou école supérieure
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Jesse Brown VA Medical Center pays non établi dans la noticeÉtablissement de santé
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Comprehensive Clinical Research pays non établi dans la noticeStructure de recherche
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Miami VA Healthcare System pays non établi dans la noticeÉtablissement de santé
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Comprehensive Transplant Center pays non établi dans la noticeInstitution
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Tarry Building 11-727 pays non établi dans la noticeInstitution
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Miami VA Medical Center pays non établi dans la noticeÉtablissement de santé
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014 pays non établi dans la noticeInstitution
Comprehensive Transplant Center — Northwestern University, Department of Microbiology-Immunology — University of Miami et Jesse Brown VA Medical Center, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.