Pro-Inflammatory Cytokines in Nasopharyngeal Aspirate From Hospitalized Children With Respiratory Syncytial Virus Infection With or Without Rhinovirus Bronchiolitis, and Use of the Cytokines as Predictors of Illness Severity
Rattachement africain : cn, cl, us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Respiratory syncytial virus (RSV) and human rhinovirus (HRV) respiratory infection in children induce production of inflammatory interleukins (ILs) in the respiratory epithelium. As IL(s) determine the severity of illness, the purpose of this study was to identify the pro-inflammatory IL(s) that could be predictor(s) of clinical severity. One hundred and fifteen patients <2 years old with bronchiolitis due to RSV and /or HRV and 38 controls were selected from a hospital and an outpatient clinic. Clinical data of all patients were recorded. Severity was defined by the number of days with oxygen need. Nasopharyngeal aspirates (NPA) were collected to perform viral diagnosis by quantitative reverse transcription and polymerase chain reaction (qRT-PCR) and to quantify ILs: TNF-α, IL-10, IL-6, IL-1β, and IL-8, by flow cytometry. Simple and multiple regression and receiver operating characteristic (ROC) curves were used for statistical analysis. Of the patients selected 60 were single RSV, 28 RSV associated to HRV, and 27 single HRV. All patients (115) showed significantly higher IL levels when compared with controls. Levels of IL-6, IL-1β, and IL-8 detected in NPA from RSV single and associated to HRV were significantly higher than HRV infected and positively associated with days requiring O2.Levels of IL-6, IL-1β, and IL-8 detected in NPA from patients infected with RSV only or with both RSV and HRV are increased, and any of those 3 cytokines may have a predictive value for the number of days with need of supplemental oxygen.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Pro-Inflammatory Cytokines in Nasopharyngeal Aspirate From Hospitalized Children With Respiratory Syncytial Virus Infection With or Without Rhinovirus Bronchiolitis, and Use of the Cytokines as Predictors of Illness Severity
- Date Crossref
- 01/09/2015
- Éditeur
- Ovid Technologies (Wolters Kluwer Health)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Puer University pays non établi dans la noticeUniversité ou école supérieure
-
University of Chile pays non établi dans la noticeUniversité ou école supérieure
-
Catholic University of America pays non établi dans la noticeUniversité ou école supérieure
-
Faculty of Medicine From the Institute of Biomedical Sciences pays non établi dans la noticeUniversité ou école supérieure
-
(MRB) pays non établi dans la noticeInstitution
-
(PU) pays non établi dans la noticeInstitution
-
Faculty of Sciences Department of Immunology pays non établi dans la noticeUniversité ou école supérieure
-
Catholic University (GV) Department of Public Health pays non établi dans la noticeUniversité ou école supérieure
-
and Family Health Center “Agustin Cruz Melo” pays non établi dans la noticeÉtablissement de santé
Puer University, University of Chile et Catholic University of America, avec 6 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.