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2013 article

Small RNAs landscape (sRNAome) of soybean ['Glycine max' (l.)]: Biogenesis, vital functions and potential applications

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Small non coding RNAs (ncRNAs) are characterized by 20-30 nucleotides (nt) long RNA molecules that have emerged as negative regulators of gene expression both at transcriptional and post-transcriptional stages in eukaryotic organisms. Small RNA (sRNA) repertoire of soybean arises from endogenous or exogenous, duplex RNA or single-stranded RNA and is characterized with perfect or imperfect hairpin (stem-loop) structures. The diversity of soybean small RNA (sRNA) population is enormous encompassing microRNAs (miRNAs) and diverse class of small interfering RNAs (siRNAs). sRNAs entail dedicated cellular machinery for their biogenesis, mode of action and are expressed in response to various environmental or developmental circumstances. The scientific understanding on the biological role of plant sRNA metabolism has indeed resulted in the exploitation of sRNA mediated gene suppression. In recent years, sRNA induced gene suppression has been shown to be an effective tool for modulating the expression levels of several genes to develop desired soybean phenotype and also in targeted down regulation of genes in functional genomics studies.

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Sujets associés

Plant Molecular Biology ResearchPlant Genetic and Mutation StudiesPlant nutrient uptake and metabolism

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