Jacobs Journal of Aquaculture and Research Immunological Responses in the Liver, Kidney, and Spleen of Atlantic Halibut Infect- ed with atypical Aeromonas salmonicida using cDNA microarrays

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We investigated the differential gene expression within Atlantic halibut (Hippoglossus hippoglossus) in response to atypical A. salmonicida infection using a 381 cDNA microarray. Atlantic halibut were infected with atypical A. salmonicida strain 0203 and held at 10-12 o C. Liver, kidney and spleen tissues were collected at 21 days post infection (DPI), and total RNA was isolated for microarray analysis. Of the 381 genes studied on the microarray, we identified a total of 169 differentially expressed (2-fold change, p<0.05) genes that were in at least one of the 3 fish samples: 46 in liver, 87 in kidney and 94 in spleen. Gene expressions of 3 fish were highly variable between fish at a mid-infection time point. Of the 46 differentially expressed genes in the liver, 28 were significantly up-regulated: betaine-homocysteine S-methyltransferase and halibut hepcidin type I were the most prominent. In the kidneys and spleens of fish 2 and 3, a greater number of adaptive immune related genes were significantly up-regulated compared to fish 1, including major histocompatibility class I receptor (MHC I) and MHC II, MHC II-associated invariant chain, proteosome activator subunit 2, heat shock protein (HSP) 70 and HSP90, t-complex polypeptide 1, CD 53-like antigen and CD 63-like antigen. Our results indicate that a wide range of functional genes involved in adaptive immune systems of Atlantic halibut was differentially expressed at 21 DPI. Especially, genes involved in antigen-processing and presenting pathways were significantly up-regulated at mid-infection time point.

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