Application of Cydia pomonella expressed sequence tags: Identification and expression of three general odorant binding proteins in codling moth
Rattachement africain : us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
The codling moth, Cydia pomonella, is one of the most important pests of pome fruits in the world, yet the molecular genetics and the physiology of this insect remain poorly understood. A combined assembly of 8 341 expressed sequence tags was generated from Roche 454 GS-FLX sequencing of eight tissue-specific cDNA libraries. Putative chemosensory proteins (12) and odorant binding proteins (OBPs) (18) were annotated, which included three putative general OBP (GOBP), one more than typically reported for other Lepidoptera. To further characterize CpomGOBPs, we cloned cDNA copies of their transcripts and determined their expression patterns in various tissues. Cloning and sequencing of the 698 nt transcript for CpomGOBP1 resulted in the prediction of a 163 amino acid coding region, and subsequent RT-PCR indicated that the transcripts were mainly expressed in antennae and mouthparts. The 1 289 nt (160 amino acid) CpomGOBP2 and the novel 702 nt (169 amino acid) CpomGOBP3 transcripts are mainly expressed in antennae, mouthparts, and female abdomen tips. These results indicate that next generation sequencing is useful for the identification of novel transcripts of interest, and that codling moth expresses a transcript encoding for a new member of the GOBP subfamily.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Application of <i>Cydia pomonella</i> expressed sequence tags: Identification and expression of three general odorant binding proteins in codling moth
- Date Crossref
- 06/12/2012
- Éditeur
- Wiley
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Agricultural Research Service pays non établi dans la noticeOrganisme public
-
Iowa State University Corn Insect and Crop Genetics Research Unit Genetics Laboratory pays non établi dans la noticeUniversité ou école supérieure
-
Washington State University Department of Horticulture and Landscape Architecture pays non établi dans la noticeUniversité ou école supérieure
-
USDA‐ARS Yakima Agricultural Research Laboratory Wapato WA 98951 USDA-ARS pays non établi dans la noticeStructure de recherche
Agricultural Research Service, Corn Insect and Crop Genetics Research Unit Genetics Laboratory — Iowa State University et Department of Horticulture and Landscape Architecture — Washington State University, avec 1 autre affiliation.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.