False-Positive Results in a Recombinant Severe Acute Respiratory Syndrome-Associated Coronavirus (SARS-CoV) Nucleocapsid-Based Western Blot Assay Were Rectified by the Use of Two Subunits (S1 and S2) of Spike for Detection of Antibody to SARS-CoV
Rattachement africain : fr, cn, es. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
To evaluate the reactivity of the recombinant proteins expressed in Escherichia coli strain BL21(DE3), a Western blot assay was performed by using a panel of 78 serum samples obtained, respectively, from convalescent-phase patients infected with severe acute respiratory syndrome-associated coronavirus (SARS-CoV) (30 samples) and from healthy donors (48 samples). As antigen for detection of SARS-CoV, the nucleocapsid protein (N) showed high sensitivity and strong reactivity with all samples from SARS-CoV patients and cross-reacted with all serum samples from healthy subjects, with either those obtained from China (10 samples) or those obtained from France (38 serum samples), giving then a significant rate of false positives. Specifically, our data indicated that the two subunits, S1 (residues 14 to 760) and S2 (residues 761 to 1190), resulted from the divided spike reacted with all samples from SARS-CoV patients and without any cross-reactivity with any of the healthy serum samples. Consequently, these data revealed the nonspecific nature of N protein in serodiagnosis of SARS-CoV compared with the S1 and S2, where the specificity is of 100%. Moreover, the reported results indicated that the use of one single protein as a detection antigen of SARS-CoV infection may lead to false-positive diagnosis. These may be rectified by using more than one protein for the serodiagnosis of SARS-CoV.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- False-Positive Results in a Recombinant Severe Acute Respiratory Syndrome-Associated Coronavirus (SARS-CoV) Nucleocapsid-Based Western Blot Assay Were Rectified by the Use of Two Subunits (S1 and S2) of Spike for Detection of Antibody to SARS-CoV
- Date Crossref
- 01/03/2006
- Éditeur
- American Society for Microbiology
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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bioMérieux (France) pays non établi dans la noticeEntreprise
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Chinese Center For Disease Control and Prevention pays non établi dans la noticeOrganisme public
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National Institute for Communicable Disease Control and Prevention pays non établi dans la noticeOrganisme public
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Universidad de Granada pays non établi dans la noticeUniversité ou école supérieure
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Emerging Pathogens Department of bioMérieux pays non établi dans la noticeInstitution
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State Key Laboratory for Infectious Disease Prevention and Control (China CDC) pays non établi dans la noticeStructure de recherche
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University of Granada Biotechnology Institute pays non établi dans la noticeUniversité ou école supérieure
bioMérieux (France), Chinese Center For Disease Control and Prevention et National Institute for Communicable Disease Control and Prevention, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.