The NOTCH pathway contributes to cell fate decision in myelopoiesis
Rattachement africain : gb, us. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
BACKGROUND: Controversy persists regarding the role of Notch signaling in myelopoiesis. We have used genetic approaches, employing two Notch zebrafish mutants deadly seven (DES) and beamter (BEA) with disrupted function of notch1a and deltaC, respectively, and Notch1a morphants to analyze the development of leukocyte populations in embryonic and mature fish. DESIGN AND METHODS: Myelomonocytes were quantified in early embryos by in situ hybridization using a myeloper-oxidase (mpx) probe. Morpholinos were used to knock down expression of Notch1a or DeltaC. Wound healing assays and/or flow cytometry were used to quantify myelomonocytes in 5-day post-fertilization (dpf) Notch mutants (BEA and DES), morphants or pu.1:GFP, mpx:GFP and fms:RFP transgenic embryos. Flow cytometry was performed on 2-3 month old mutant fish. RESULTS: The number of mpx(+) cells in embryos was reduced at 48 hpf (but not at 26 hpf) in DES compared to WT. At 5 dpf this was reflected by a reduction in the number of myelomonocytic cells found at the wound site in mutants and in Notch1a morphants. This was due to a reduced number of myelomonocytes developing rather than a deficit in the migratory ability since transient inhibition of Notch signaling using DAPT had no effect. The early deficit in myelopoiesis was maintained into later life, 2-3 month old BEA and DES fish having a decreased proportion of myelomonocytes in both the hematopoietic organ (kidney marrow) and the periphery (coelomic cavity). CONCLUSIONS: Our results indicate that defects in Notch signaling affect definitive hematopoiesis, altering myelopoiesis from the early stages of development into the adult.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- The NOTCH pathway contributes to cell fate decision in myelopoiesis
- Date Crossref
- 20/09/2011
- Éditeur
- Ferrata Storti Foundation (Haematologica)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
-
Imperial College London Department of Life Sciences pays non établi dans la noticeUniversité ou école supérieure
-
Harvard University pays non établi dans la noticeUniversité ou école supérieure
-
Dana-Farber Cancer Institute Stem Cell Program and Division of Haematology/Oncology pays non établi dans la noticeStructure de recherche
-
Harvard Stem Cell Institute pays non établi dans la noticeStructure de recherche
-
University of Sheffield MRC Centre for Developmental and Biomedical Genetics pays non établi dans la noticeUniversité ou école supérieure
-
Faculty of Medicine NHLI Division pays non établi dans la noticeUniversité ou école supérieure
-
Medical Sciences pays non établi dans la noticeInstitution
Department of Life Sciences — Imperial College London, Harvard University et Stem Cell Program and Division of Haematology/Oncology — Dana-Farber Cancer Institute, avec 4 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.