Occurrence of Coxiella burnetii and Chlamydiales species in abortions of domestic ruminants and in wild ruminants in Hungary, Central Europe
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Le résumé fourni par la source
Coxiella burnetii and certain members of the Chlamydiales order are zoonotic, intracellular, Gram-negative bacteria, with abortigenic potential in ruminants. These pathogens have a broad host range and worldwide geographical distribution. The current study aimed to reveal the importance of C. burnetii and Chlamydiales spp. in abortions in domestic ruminants and their occurrence in wild ruminants with real-time polymerase chain reaction (qPCR) assays, histology, and immunohistochemical staining (IHC). From the 111 abortion cases of domestic ruminants examined, C. burnetii was detected in 33 placenta samples (cattle, n = 22; sheep, n = 10; goat, n = 1), and members of the Chlamydiales order were detected in 32 placenta samples (cattle, n = 14; sheep, n = 16; goat, n = 2) using qPCR. Coinfection with both C. burnetii and Chlamydiales spp. were identified in 12 cases (cattle, n = 3; sheep, n = 8; goat, n = 1) out of the qPCR-positive samples. The presence of the relevant antigen was confirmed by IHC in 20 cases (C. burnetii, n = 2, in sheep; Chlamydiaceae, n = 17, in sheep [n = 15] and goat [n = 2]; and both pathogens in 1 sheep). Coxiella burnetii was identified in 2.2% (2/91) of the wild ruminants, but the samples were negative by IHC. Uncultured Chlamydiales spp. were detected in 4.4% (4/91) of the placenta samples by qPCR. In conclusion, Q fever is widespread among domestic ruminants in Hungary, and, in several cases, C. burnetii was implicated as the primary cause of abortions. Waddlia chondrophila, Parachlamydia spp., and uncultured Chlamydiales spp. were present only sporadically in samples from cattle and wild ruminants.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Occurrence of <i>Coxiella burnetii</i> and <i>Chlamydiales</i> species in abortions of domestic ruminants and in wild ruminants in Hungary, Central Europe
- Date Crossref
- 01/03/2015
- Éditeur
- SAGE Publications
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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University of Zurich Institute of Veterinary Pathology pays non établi dans la noticeUniversité ou école supérieure
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National Food Chain Safety Office pays non établi dans la noticeOrganisme public
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HUN-REN Veterinary Medical Research Institute pays non établi dans la noticeStructure de recherche
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University of Kaposvár pays non établi dans la noticeUniversité ou école supérieure
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HUN-REN Centre for Agricultural Research pays non établi dans la noticeStructure de recherche
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Hungarian Academy of Sciences pays non établi dans la noticeOrganisme public
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Institute for Veterinary Medical Research pays non établi dans la noticeStructure de recherche
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Kaposvár University pays non établi dans la noticeUniversité ou école supérieure
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Veterinary Diagnostic Directorate pays non établi dans la noticeInstitution
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Veterinary practitioners pays non établi dans la noticeInstitution
Institute of Veterinary Pathology — University of Zurich, National Food Chain Safety Office et HUN-REN Veterinary Medical Research Institute, avec 7 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.