Increased Expression of the Glucocorticoid Receptor β in Infants With RSV Bronchiolitis
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Le résumé fourni par la source
OBJECTIVES: The majority of studies on glucocorticoid treatment in respiratory syncytial virus (RSV) bronchiolitis concluded that there are no beneficial effects. We hypothesized that RSV-infected patients may have an increased glucocorticoid receptor (GR) β expression, the isoform that is unable to bind cortisol and exert an antiinflammatory action. METHODS: By using real-time polymerase chain reaction, we studied the expression of α and β GR in the peripheral blood mononuclear cells obtained from 49 RSV-infected infants (<1 year of age) with severe (n = 29) and mild to moderate (n = 20) illness. In plasma, we analyzed the level of cortisol by radioimmunoassay and inflammatory cytokines interleukin (IL)-10, IL-6, tumor necrosis factor-α, IL-1β, IL-8, IL-12p70, IL-2, IL-4, IL-5, interferon-γ, and IL-17 by cytometric beads assay. Statistical analysis was performed by nonparametric analysis of variance. RESULTS: We found a significant increase of β GR expression in patients with severe illness compared with those with mild disease (P < .001) and with a group of healthy controls (P < .01). The α:β GR ratio decreased significantly in infants with severe disease compared with those with mild illness (P < .01) and with normal controls (P < .001). The expression of β GR was positively correlated with the clinical score of severity (r = .54; P < .0001). CONCLUSIONS: The decrease of the α:β GR ratio by an increase of β receptors expression is related to illness severity and may partly explain the insensitivity to corticoid treatment in RSV-infected infants. The increased expression of β GR could be a marker of disease severity.
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Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé ; titre concordant.
- Titre Crossref
- Increased Expression of the Glucocorticoid Receptor β in Infants With RSV Bronchiolitis
- Date Crossref
- 01/10/2012
- Éditeur
- American Academy of Pediatrics (AAP)
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Apath (United States) pays non établi dans la noticeEntreprise
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Research Institute of General Pathology and Pathophysiology pays non établi dans la noticeStructure de recherche
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University of Chile pays non établi dans la noticeUniversité ou école supérieure
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Pathophysiology Program pays non établi dans la noticeInstitution
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Faculty of Medicine Department of Pediatrics pays non établi dans la noticeUniversité ou école supérieure
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Centro de Salud Familiar Agustin Cruz Melo pays non établi dans la noticeInstitution
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Faculty of Sciences Biology Department pays non établi dans la noticeUniversité ou école supérieure
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Virology Program pays non établi dans la noticeInstitution
Apath (United States), Research Institute of General Pathology and Pathophysiology et University of Chile, avec 5 autres affiliations.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.