Use of Recombinant ESAT-6:CFP-10 Fusion Protein for Differentiation of Infections of Cattle by Mycobacterium bovis and by M. avium subsp. avium and M. avium subsp. paratuberculosis
Rattachement africain : us, Tanzanie. Niveau de preuve : code pays fourni par la source.
Le résumé fourni par la source
Immunological diagnosis of Mycobacterium bovis infection of cattle is often confounded by cross-reactive responses resulting from exposure to other mycobacterial species, especially Mycobacterium avium. Early secretory antigenic target 6 (ESAT-6) and culture filtrate protein 10 (CFP-10) are dominant gamma interferon (IFN-gamma)-inducing antigens of tuberculous mycobacteria, and they are absent from many environmental nontuberculous mycobacteria. Because M. avium exposure is the primary confounding factor in the diagnosis of M. bovis-infected animals, in vitro responses to a recombinant ESAT-6:CFP-10 (rESAT-6:CFP-10) fusion protein by blood leukocytes from cattle naturally exposed to M. avium or experimentally challenged with Mycobacterium avium subsp. avium or Mycobacterium avium subsp. paratuberculosis were compared to responses by M. bovis-infected cattle. Responses to heterogeneous mycobacterial antigens (i.e., purified protein derivatives [PPDs] and whole-cell sonicates [WCSs]) were also evaluated. Tumor necrosis factor alpha (TNF-alpha), IFN-gamma, and nitric oxide responses by M. bovis-infected cattle to rESAT-6:CFP-10 exceeded (P < 0.05) the corresponding responses by cattle naturally sensitized to M. avium. Experimental infection with M. bovis, M. avium, or M. avium subsp. paratuberculosis induced significant (P < 0.05) IFN-gamma and nitric oxide production to WCS and PPD antigens, regardless of the mycobacterial species used for the preparation of the antigen. Responses to homologous crude antigens generally exceeded responses to heterologous antigens. Nitric oxide and IFN-gamma responses to rESAT-6:CFP-10 by blood leukocytes from M. bovis-infected calves exceeded (P < 0.05) the corresponding responses of noninfected, M. avium-infected, and M. avium subsp. paratuberculosis-infected calves. Despite the reported potential for secretion of immunogenic ESAT-6 and CFP-10 proteins by M. avium and M. avium subsp. paratuberculosis, it appears that use of the rESAT-6:CFP-10 fusion protein will be useful for the detection of tuberculous cattle in herds with pre-existing sensitization to M. avium and/or M. avium subsp. paratuberculosis.
Ce résumé expose les affirmations des auteurs. BNTIC ne l’interprète pas comme une validation indépendante des résultats.
Le contrôle bibliographique ouvert
DOI retrouvé dans Crossref DOI retrouvé, mais le titre doit être comparé manuellement.
- Titre Crossref
- Use of Recombinant ESAT-6:CFP-10 Fusion Protein for Differentiation of Infections of Cattle by <i>Mycobacterium bovis</i> and by <i>M. avium</i> subsp. <i>avium</i> and <i>M. avium</i> subsp. <i>paratuberculosis</i>
- Date Crossref
- 01/07/2004
- Éditeur
- American Society for Microbiology
- Type
- journal-article
Ce recoupement confirme des métadonnées liées au DOI. Il ne confirme ni la méthode ni les conclusions de l’étude, et il ne compte pas comme une seconde source scientifique indépendante.
Où se fait cette recherche
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Animal and Plant Health Inspection Service pays non établi dans la noticeOrganisme public
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Agricultural Research Service pays non établi dans la noticeOrganisme public
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Ministry of Livestock and Fisheries Tanzanie (code pays fourni par la source)Organisme public
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National Animal Disease Center pays non établi dans la noticeStructure de recherche
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The University of Texas Medical Branch at Galveston Sealy Center for Vaccine Development pays non établi dans la noticeUniversité ou école supérieure
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Bacterial Diseases of Livestock Research Unit pays non établi dans la noticeStructure de recherche
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Department of Pediatrics pays non établi dans la noticeInstitution
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National Veterinary Services Laboratory Mycobacteria and Brucella Section pays non établi dans la noticeStructure de recherche
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Periparturient Diseases of Cattle Research Unit pays non établi dans la noticeStructure de recherche
Animal and Plant Health Inspection Service, Agricultural Research Service et Ministry of Livestock and Fisheries (Tanzanie), avec 6 autres affiliations. Pays d’affiliation : Tanzanie.
Une affiliation ne permet pas de déduire la nationalité d’un auteur.